马铃薯晚疫病菌高灵敏性PCR检测技术研究

Studies on High Sensibility PCR Detection Technology for Potato Late Blight

由致病疫霉菌引起的马铃薯晚疫病是马铃薯生长期和储藏期的一种毁灭性病害。PCR检测技术已成为检测晚疫病致病菌的一种常规方法。本研究利用9对国内外已发表的晚疫病检测引物,通过对致病疫霉菌和其他5种常见的侵染马铃薯的病原真菌进行PCR检测,筛选出Primer-4-2和Primer-5对晚疫病菌的特异性较好,而其他7对引物特异性较差或不具有特异性。在对带病薯块、植株及组培苗的样品检测中,引物Primer-4-2特异性优于Primer-5,能够实现对不同晚疫病样品的检测,其引物灵敏度检测为10 pg/μL。

Potato late blight is a devastating disease which caused by Phytophthora infestans during the growth andstorage period. Up to now, PCR detection technology has become a routine way to detect the pathogen. In the presentstudy, nine detecting primers of Phytophthora infestans which have been published in China and abroad were usedto detect the specificity of Phytophthora infestans with other 5 common pathogens which also could infect potato byPCR technology. The results showed that both Primer-4-2 and Primer-5 have a good specific to distinguish thepathogen, and the specificity of other 7 primers was poor or not. Compared with these two primers, the specificity ofprimer-4-2 was better than primer-5, and it could give a good detection on different diseased samples included tu-bers, plant tissues and plantlets. The sensitivity of primer-4-2 reached to 10 pg/μL.