摘要
目的：分析地肤病毒病的病原。方法：在生物学检测的基础上，提取病毒双链RNA（dsRNA），合成单链cDNA后经多重PCR扩增、测定序列并分析，对地肤病毒病进行了鉴定。结果：有明显花叶症状的地肤由蚕豆萎蔫病毒2号（Broad bean wilt virus 2，BBWV2）侵染所致。将地肤分离物（Isolate-DF）中部分病毒序列进行比对分析，发现其与已报道的15个不同来源BBWV2序列的同源性为79.9%～88.0%。结论：通过分子鉴定，首次证实我国药用植物地肤上发生的花叶病毒病系由BBWV2侵染所致。
Objective：Analyze the pathogeny on the Kochia scoparia virus disease. Method：The Kochia scoparia virus was identified,the virus double-stranded RNA （dsRNA）was extracted,and the single cDNA was synthesized using the multiplex PCR amplification method,and the target sequence was analyzed. Result：The Mosaic symptoms of K. scoparia were caused by the infection of Broad bean wilt virus 2 （Broad bean wilt virus 2,BBWV2）. Sequence homology analysis revealed that the partial virus sequence from Isolate-DF of K. scopria showed 79.9%~88.0% homology with other 15 reported BBWV sequences. Conclusion：The results of nucleotides homology analyzing firstly confirmed that the Mosaic virus disease of medicinal plant K.scopria was caused by BBWV2 in china.
出处
《山西中医学院学报》
2016年第4期25-27,35,共4页
Journal of Shanxi College of Traditional Chinese Medicine
关键词
地肤
生物学检测
多重PCR扩增
蚕豆萎蔫病毒2号
Kochia scopria
Biological detection
the multiplex PCR amplification
Broad bean wilt virus 2
