低氧联合TNF-α对人肺微血管内皮细胞氧化应激及凋亡的影响

Influence of Hypoxia Combined with Tumor Necrosis Factor-αon the Apoptosis and Oxidative Stress in Human Pulmonary Microvascular Endothelial Cells

目的探讨低氧联合肿瘤坏死因子α(TNF-α)对人肺微血管内皮细胞(HPMVECs)氧化应激及凋亡的影响。方法低氧6、12、24h及10、20、50、100ng/mL TNF-α分别处理HPMVECs,使用MTT比色法检测各组的细胞活性;选用最佳的低氧时间及TNF-α剂量作为联合干预组,检测各组上清液的丙二醛(MDA)含量、乳酸脱氢酶(LDH)及超氧化物歧化酶(SOD)活力及细胞凋亡率。结果低氧及TNF-α联合干预组的MDA含量、LDH活力及细胞凋亡率明显高于单独作用组(均P<0.05),而SOD活力低于单独作用组(均P<0.05);且低氧24h+TNF-α100ng/mL联合干预组的细胞凋亡率最高(P<0.05)。结论低氧联合TNF-α作用于HPMVECs后,细胞的氧化应激及凋亡率明显增加。

Objective To explore the influence of hypoxia combined with tumor necrosis factor-α（TNF-α）on the apoptosis and oxidative stress in human pulmonary microvascular endothelial cells（HPMVECs）.Methods HPMVECs were treated by hypoxia for 6,12,or 24 hand then with TNF-αat concentration of 10,20,50,or 100ng/mL.MTT was used to detect the cell viability in each group.The contents of malondialdehyde（MDA）,activities of lactate dehydrogenase（LDH）and superoxide dismutase（SOD）,and the apoptosis rate were determined in the combined treatment group which had the optimal treatment time of hypoxia and concentration of TNF-α.Results The contents of MDA,the activities of LDH and the apoptosis rate were significantly higher and the activity of SOD was significantly lower in the combined treatment group than in single treatment group（P〈0.05 for all）.The apoptosis rate was highest in the 24h hypoxia＋100ng/mL TNF-αtreatment group.Conclusion The oxidative stress and apoptosis rate are significantly increased in HPMVECs treated with hypoxia and TNF-αcombined.