miR-19b、miR-29c及其靶基因表达在鼻咽癌发病中的作用

Role of miR-19b,miR-29c and Their Target Genes in Nasopharyngeal Carcinoma

目的探讨miR-19b、miR-29c及其靶基因表达在鼻咽癌发病中的作用。方法选择2012年7月至2015年7月武汉大学中南医院收治的70例鼻咽癌患者作为鼻咽癌组,57例慢性鼻咽炎患者作为鼻咽炎组,另选择同期在医院体检的健康人群40例作为对照组,抽取3组血液样本,采用荧光定量PCR检测3组血清miR-19b、miR-29c表达;收集鼻咽癌组、鼻咽炎组组织样本,采用Western blot检测靶基因SOCS-1、COL1A-1蛋白表达水平。结果以β-actin作为内参照,鼻咽癌组血清miR-19b、miR-29c相对表达量分别为(374.91±14.16)和(6.84±0.27),鼻咽炎组分别为(171.53±7.82)和(2.14±0.07),对照组分别为(168.95±8.05)和(2.08±0.05),鼻咽癌组血清miR-19b、miR-29c相对表达量显著高于鼻咽炎组和对照组(均P<0.05),鼻咽炎组和对照组miR-19b、miR-29c表达量比较差异无统计学意义(均P>0.05)。Western blot检测结果显示,鼻咽癌组SOCS-1蛋白表达显著低于鼻咽炎组(P<0.05),鼻咽炎组与鼻咽癌组COL1A-1蛋白表达比较差异无统计学意义(P>0.05)。相关性分析显示,血清miR-19b与SOCS-1呈负相关关系(r=-0.573,P<0.01);miR-29c与COL1A-1无相关性(r=0.148,P=0.291)。结论鼻咽癌组血清miR-19b、miR-29c表达显著升高,可以作为鼻咽癌诊断的潜在标志物;miR-19b可能通过调节靶基因SOCS-1参与了鼻咽癌的发生。

Objective To explore the role of miR-19 b,miR-29 cand their target genes in the development of nasopharyngeal carcinoma.Methods A total of 70 nasopharyngeal carcinoma patients treated in Zhongnan Hospital of Wuhan University from July 2012 to July 2015 were selected as nasopharyngeal carcinoma group,57 patients with chronic nasopharyngitis as nasopharyngitis group,and another 40 healthy individuals as control group.Blood samples were collected from the three groups.The expression of miR-19 band miR-29 cwas detected by real-time quantitative PCR in the three groups.The expression levels of target genes SOCS-1 and COL1A-1 protein were detected by Western blotting in nasopharyngeal carcinoma and nasopharyngitis tissues.Results Withβ-actin as an internal reference,the relative expression levels of miR-19 band miR-29cwere（374.91±14.16）and（6.84±0.27）in nasopharyngeal carcinoma group,（171.53±7.82）and（2.14±0.07）in nasopharyngitis group,and（168.95±8.05）and（2.08±0.05）in control group.They were significantly increased in nasopharyngeal carcinoma group as compared with those in nasopharyngitis group and control group（P〈0.05 for all）.There was no significant difference in the expression of miR-19b and miR-29c between nasopharyngitis group and control group（P〉0.05）.Western blotting showed that the expression of SOCS-1 was much lower in nasopharyngeal carcinoma group than that in nasopharyngitis group（P〈0.05）.There was no significant difference in the expression of COL1A-1 protein between the nasopharyngeal carcinoma group and the nasopharyngitis group（P〉0.05）.Correlation analysis showed that serum miR-19 bwas negatively correlated with SOCS-1（r=-0.573,P〈0.01）,and miR-29c had no correlation with COL1A-1（r=0.148,P=0.291）.Conclusion The levels of serum miR-19b and miR-29c were significantly increased in patients with nasopharyngeal carcinoma,which suggested that serum miR-19b and miR-29c may be used as a potential marker for diagnosis of nasopharyngeal carcinoma.miR-19b may be involved in the occurrence of nasopharyngeal carcinoma by regulating the target gene SOCS-1.