4种解释系统对HIV-1基因型耐药解释相关性评价

Evaluation of the Correlation of Four System for Testing HIV-1 Genotype Drug Resistance

目的:比较HIVDB(7.0.1)、ANRS(25_09/2015)、REGA(9.1.0)和HIV GRADE(12/2015)4种解释系统对HIV-1基因型耐药结果解释的一致性。方法:选取我国3种主要HIV-1流行亚型和流行重组模式(B’、CRF01_AE和CRF07_BC),每种选取200条序列,共600条序列。分别用4种解释系统进行耐药结果分析,分为耐药(R)、中度耐药(I)和敏感(S)3个水平。对4种结果进行统计学分析。结果:4种系统基因型耐药解释结果比较相关性较高(r均>0.57,P均<0.01)。针对亚型来说,CRF07_BC的解释结果相关性最高,其次为B’亚型,CRF01_AE略低。结论:对于我国主要流行的HIV来说,4种解释系统对11种常用的抗病毒治疗药物耐药结果的解释具有较好的一致性。

Objective：To compare the correlation of four drug-resistance interpretation system including HIVDB, ANRS, REGA and HIV GRADE for testing HIV-1 genotype drug resistance. Methods：Trendy subtypes and restructuring model of HIV-1 including B’,CRF01AE and CRF07BC were selected,each genotype was 200 series,600 samples were analyzed by four drug-resistance interpretation system, the results were divided into three levels including resistance （R）, possible drug-resistance （I） and susceptible （S）. Results：Four drug-resistance interpretation system had a high correlation in genotypic drug-resistance consequence （rs〉0.57, P〈0.01）, CRF07BC had the highest correlation, followed by subtype B, CRF01AE was slightly lower.Conclusion：Four drug-resistance interpretation system has a high correlation in analyzing antiviral drug resistance for major epidemic of HIV in China.