肾母细胞瘤中ERCC1、TUBB3、TOP2A mRNA的表达及临床意义

Expression and clinical significance of ERCC1,TUBB3 and TOP2A mRNA in nephroblastoma

目的探讨核苷酸切除修复交叉互补基因(excision repair cross complementation 1,ERCC1)、3型β微管蛋白编码基因(ClassⅢβ-tubulin,TUBB3)和DNA拓扑异构酶Ⅱα(TopoisomeraseⅡA,TOP2A)mRNA在小儿肾母细胞瘤组织中的表达及临床意义。方法收集18例肾母细胞瘤组织标本及14例癌旁组织标本,通过分支-DNA液相芯片法检测标本中ERCC1、TUBB3、TOP2A mRNA的表达水平,并分析上述基因表达与肾母细胞瘤临床病理的关系。结果肾母细胞瘤组织中ERCC1、TUBB3和TOP2A高表达率分别为44.4%、50.0%和66.7%,均显著高于癌旁组织的7.1%、7.1%和14.3%,差异有统计学意义(P<0.05)。ERCC1高表达率与肾母细胞瘤临床分期和病理类型有关(P<0.05),TOP2A高表达率与肾母细胞瘤临床分期相关(P<0.05)。结论小儿肾母细胞瘤组织中ERCC1、TUBB3和TOP2A mRNA表达高于癌旁组织,可能参与肾母细胞瘤的发生发展过程。

Objective To investigate the expression and clinical significance of excision repair cross complementation 1（ERCC1）,Class Ⅲ β-tubulin（TUBB3）and topoisomerase ⅡA（TOP2A）in nephroblastoma.Methods The expression levels of ERCC1,TUBB3 and TOP2 A in 18 cases of nephroblastoma tissue and 14 cases of peritumoral tissue were detected by branch-DNA liquid chip technology.The relationship of the expressions of the above genes with the clinicopathologic characteristics of nephroblastoma was analyzed.Results The high expression rates of ERCC1,TUBB3 and TOP2 A in nephroblastoma tissues（44.4%,50.0%,66.7%） were significantly higher than those in peritumoral tissues（7.1%,7.1% and 14.3%）（P〈0.05）.The high expression of ERCC1 was correlated with the clinical stage and histopathologic type（P〈0.05）.TOP2 A was correlated with the clinical stage（P〈0.05）.Conclusion The expressions of ERCC1,TUBB3 and TOP2 A were higher in nephroblastoma than in normal renal tissue.High expressions of ERCC1,TUBB3 and TOP2 A may play an important role in carcinogenesis and progression of nephroblastoma.