不同培养方法对牙周膜细胞生物学特性的影响

Effect of culture methods on the characteristics of periodontal ligament cells

目的探讨酶消化法和组织块法是否影响牙周膜细胞的特性。方法培养牙周膜细胞,检测克隆形成能力及细胞周期分析,Stro-1的表达,实时定量RT-PCR检测。结果酶消化法培养的PDLCs+G2M明显高于组织块法的PDLC;酶消化法培养的PDLC克隆形式能力明显高于组织块法培养的PDLC;酶消化法培养的PDLC Stro-1的表达明显高于组织块法培养的PDLC;酶消化法培养的PDLC成骨标志物的表达明显高于组织块法培养的PDLC。酶消化法培养的牙周膜细胞具有更强的增殖能力和间充质干细胞含量更多,成骨标志物表达更强。结论酶消化法和组织块法影响牙周膜细胞的某些特性。

Objective To study whether tissue block method and enzymic digestion can influence periodon- tal ligament cell. Methods Periodontal ligament cells （PDLCs） were isolated and Colony-forming assay and cell cycle analysis were conducted. Real-time RT-PCR and the expression of stro-1 were evaluated. Results PDLCS＋G2M, PDLC cloning ability, PDLC Stro-l＂s expression and PDLC osteogenesis markers＂ expression cultured by enzymic digestion method were significant higher than PDLC cultured by tissue block method. PDLCs cultured by enzymic digestion method had stronger proliferation ability, more mesenchymal stem cells and stronger expression osteogenesis markers. Conclusions Tissue block method and enzymic digestion could.influence biologic characteristics of PDLCs.