不同放疗剂量对人唾液腺腺样囊性癌细胞生物学行为的影响

Effect of different doses of radiation on human salivary gland adenoid cystic carcinoma cells

目的 :探讨不同放疗剂量对人唾液腺腺样囊性癌(SACC-83、SACC-LM)细胞生物学行为的影响。方法 :采用不同放疗剂量(0、2、4、6、8、10 Gy)作用SACC-83、SACC-LM细胞并继续培养48 h,CCK-8检测细胞存活,流式细胞仪检测细胞凋亡和细胞周期,细胞划痕实验观察细胞迁移能力变化。采用SPSS19.0软件包对数据进行统计学分析。结果:放疗对SACC-LM细胞的细胞存活率、细胞凋亡率、异倍体、细胞迁移力的影响较SACC-83显著(P<0.05);与其他放疗剂量相比,SACC-83细胞在6 Gy剂量照射下细胞存活率最低,细胞凋亡比例及异倍体比例最高,细胞迁移能力最弱。结论:SACC-LM细胞放疗敏感性较SACC-83更高,SACC-83细胞对6 Gy剂量放疗最为敏感。

PURPOSE： To explore the effect of different doses of radiation on human salivary gland adenoid cystic carcinoma cells （SACC-83, SACC-LM）. METHODS： Different doses of radiation （0, 2, 4, 6, 8, 10 Gy） were applied to SACC-83, SACC-LM cells and the cells were continued to culture for 48 h. CCK-8 test, flow cytometry（FCM） and cell scratch experiment were used to observe cell proliferation, apoptosis and cell migration. SPSS19.0 software package was used for data analysis. RESULTS： The effect of radiation on SACC-LM cells survival rate, cell apoptosis, heteroploid and cell migration ability were significantly greater than that on SACC-83 cells （P〈0.05）. In SACC-83 cells, compared with other doses of radiation, 6 Gy of irradiation was the most sensitive on cell survival rate, cell apoptosis, heteroploid and cell migration ability. CONCLUSIONS： Radiation sensitivity of SACC-LM was greater than that of SACC-83 cells. With 6 Gy of radiation, changes of biology in SACC-83 cells most significant than other doses of radiation.