醋氨酚、布他比妥和咖啡因联用的镇痛作用及其急性毒性的定量分析

Quantitative analysis of analgesic effect and acute toxicity ofacetaminophen, butabital and caffeine in combination in mice

目的定量分析醋氨酚、布他比妥和咖啡因联用的镇痛作用及急性毒性变化 ,以确定合适的联用比例和联用剂量。方法采用配方均匀设计法设置7个水平联用比例和剂量 ,用小鼠甩尾法作镇痛试验 ,综合数理分析结果和专业知识 ,再经确定性试验证实 ;采用参数法分析联用的急性毒性数据。结果(1)醋氨酚40.7mg/kg,布他比妥6.4mg/kg 和咖啡因5mg/kg联用效果较好 ,其联用比例为8.1∶1.25∶1 ;(2)3药联用有协同作用 ;(3)联用的急性毒性未见增强。结论醋氨酚 ,布他比妥和咖啡因联用(8.1∶1.25∶1)镇痛效应增强 ,但毒性未增(无协同作用)

Aim To analyzed and evaluate the analgesic effect of acetaminophen (Ace), butabital (But) and caffeine (Caf) in combination . Methods According to the formula uniform design method, 7 different doses at 7 different proportions were administered (ig) to observe the analgesic effect in mice by the swirling tail method. The optimum doses and proportion in combination were obtained according to the experimental data, mathematical analysis and the special field demand, which was demonstrated by another experiments (the swirling tail method and the light-electric vibrating box method). Acute toxicity of the combination was quantitatively analyzed by the parameter method. Results The combination of Ace 40.7, But 6.4 and Caf 5 mg/kg ( proportion as 8.1∶1.25∶1) was appropriate and the acute toxicity didn't increased. Conclusion The formula uniform design method can be effectively applied to analyze the multidrug effects, but its result must take a special field demand into account, and the estimated optimum dose and proportion for combination be demonstrated by another further experiment.