不平衡支链氨基酸对胃癌细胞体外增殖和化疗敏感性的影响

EFFECT OF IMBALANCED BRANCHED-CHAIN AMINO ACIDS ON GROWTH AND CHEMOTHERAPEUTIC SENSITIVITY OF GASTRIC CANCER CELL LINE SGC-7901 IN VITRO

目的 为观察不平衡支链氨基酸对胃癌细胞体外增殖和对化疗药物5-FU、MMC和MTX敏感性的影响,为肿瘤的氨基酸失衡疗法提供实验和理论基础。方法 在不含L-Val的MEM培养基基础上,以普通MEM培养基L-Val（46mg/L）和L-Leu含量（52mg/L）为准,配制12种限制L-Val、增加L-Leu的不平衡支链氨基酸培养基,用MTT法观察其对胃癌细胞株SGC-7901体外增殖的影响和对该细胞株化疗药物敏感性的影响。结果 限制L-Val至正常的1/4对 SGC-7901细胞即有明显的抑制作用（p<0.01）,随着L-Val浓度的进一步降低,抑制作用更加显著。增加 L-Leu至正常的 4倍,可显著增强 L-Val缺乏对SGC-7901细胞的抑制作用。不平衡支链氨基酸可提高化疗药物5-FU、MMC和MTX对SGC-7901的杀伤作用。结论 不平衡支链氨基酸对胃癌细胞体外增殖有抑制作用,与化疗药物有协同抗肿瘤效应。在肿瘤的氨基酸失衡疗法中,不仅要调整某种氨基酸的绝对量,更重要的是调整该氨基酸与其他氨基酸的比例关系。

objective To observe the effect of Unbalanced branched - chain amino acids on growth and chemotherabeutic sensitivity of gastric cancer cell line SGC-7901 in vitro, and to provide laboratory data for imbalanced amino acids therapy for cancer. Methods L - valine and L - leucine of MEM culture medium were restricted and increased to serial levels respectively and used to culture gastric cancer cell line SGC-7901. The growth and chemotherapeutic sensitivity of SGC-7901 in vitro were measuredwith MTT method. Results Significant inhibition of SGC- 7901 growth presented when L - valine was restricted to less than 1/4 of normal. At same restricted level of L- valine, increasing L- leucine to 4 times improved inhibition of SGC-7901. Improved inhibition effect of chemotherapy was achieved at imbalanced branched - chain amino acids. Conclusion Imbalanced branched - chain amino acids can inhibit growth of cancer cell and have synergistic effect with chemotherapy. Proportion of amino acids is more important than absolute levels in adjuvant anti - cancer amino acids therapy.