鱼腥草挥发油对Raji细胞增殖和凋亡的影响

Effect of volatile oil from houttuyniae herba on proliferation and apoptosis of Raji cells

目的:研究鱼腥草挥发油对Raji细胞增殖与凋亡的影响。方法:采用MTT法观察不同体积分数的鱼腥草挥发油体外诱导Raji细胞24、48和72 h对细胞增殖的影响;分别采用Annexin V-FITC/PI双染法及PI单染法检测不同体积分数的鱼腥草挥发油处理Raji细胞48 h后细胞凋亡及细胞周期的改变。结果:鱼腥草挥发油诱导Raji细胞24、48和72 h的IC50(V/V)分别为0.125 80×10-3、0.099 58×10-3和0.105 22×10-3,且随鱼腥草挥发油体积分数的增加,细胞增殖抑制率及凋亡率均呈升高的趋势(P<0.05);细胞周期检测结果表明鱼腥草挥发油将细胞阻滞于G0/G1期。结论:鱼腥草挥发油能够显著抑制Raji细胞的增殖,诱导细胞凋亡;其作用机制可能与阻滞细胞G1/M转化有关。

Aim： To investigate the effect of volatile oil from houttuyniae herba（ HHO） on the proliferation and apoptosis of Raji cells. Methods： Different volume fraction of HHO was used to treat Raji cells for 24,48 and 72 h; the prolifera-tion of Raji cells was determined by MTT assay. Different volume fraction of HHO was used to treat Raji cells for 48 h; the cell apoptosis rate was measured by Annexin V-FITC / PI double staining method,and the cell cycle was observed by PI staining method. Results： The MTT results showed that IC50（ V/V） of HHO for 24,48 and 72 h treatment was 0. 125 80 ×10- 3,0. 099 58 × 10- 3and 0. 105 22 × 10- 3; with the increase of volume fraction,the inhibitory effect and apoptosis rate showed a rising trend（ P 0. 05）. The results of cell cycle detection showed that HHO was able to arrest Raji cells in G0/G1 phase. Conclusion： HHO could significantly inhibit the proliferation of Raji cells and induce cell apoptosis,and themechanism may be related to HHO＇s blocking G1/ M conversion of Raji cells.