移植物中CCR6^+及CCR7^+ T细胞数量与异基因造血干细胞移植受者急性移植物抗宿主病发生的关系

Correlation of quantity of CCR6^+ and CCR7^+ T lymphocytes in grafts with occurrence of acute graft-versus-host disease in recipients after allo-HSCT

目的:探讨移植物中表达趋化因子受体6(CCR6)或CCR7的T细胞数量与异基因造血干细胞移植后急性移植物抗宿主病(GVHD)发生的关系。方法:选取行亲缘异基因造血干细胞移植的52对供受者为研究对象,经重组人粒细胞集落刺激因子动员后,测定并比较骨髓和外周血采集物中CCR6+或CCR7+T细胞的数量,分析输注的各亚群T细胞数量与移植后受者发生Ⅱ-Ⅳ度急性GVHD的关系。结果:动员后,外周血采集物中CD4+CCR6+、CD8+CCR6+、CD4+CCR7+及CD8+CCR7+T细胞的绝对数量均较骨髓中增多(P<0.05),是骨髓中的15~25倍,但移植物中上述各亚群细胞的绝对数量不是移植后受者发生急性GVHD的危险因素(P>0.05)。结论:移植后受者急性GVHD的发生可能与移植物中CCR6+或CCR7+T细胞的数量无关。

Aim： To detect the quantity of T cell subsets expressing C-C chemokine receptor type 6（ CCR6） or CCR7 in the grafts and analyze their correlation with the occurrence of acute graft-versus-host disease（ GVHD）. Methods： Fifty-two pairs of healthy donor and their recipients with family donor allogenetic hematopoietic stem cell transplantation（ allo-HSCT） were included in this study. All the recipients were injected recombinant human granulocyte colony-stimulating factor（ rh G-CSF） for mobilizing.The quantity of T cells expressing CCR6 or CCR7 in rh G-CSF mobilized bone marrow grafts（ G-BM） and peripheral blood grafts（ G-PB） were detected and compared. The correlation of the quantity of each subset cells with the cumulative incidence of gradesⅡto Ⅳ acute GVHD in recipients was analyzed. Results： After rh G-CSF mobilization,G-PB had obviously more T cells than GBM（ P 0. 05）. CD4＋CCR6＋,CD8＋CCR6＋,CD4＋CCR7＋and CD8＋CCR7＋T cells in G-PB were approximately 15- 25times more than those in G-BM. And the absolute counts of the above T cell subsets were not risk factors for acute GVHD（ P 0.05）. Conclusion： The quantity of T cell subsets may not be related to the occurrence of acute GVHD.