紫花牡荆素对人宫颈癌细胞凋亡和侵袭迁移的影响

Effects of casticin on apoptosis, migration and invasion in human cervical cancer cells

目的观察紫花牡荆素(CAT)对人宫颈癌He La和Si Ha细胞凋亡和侵袭转移的影响。方法不同浓度CAT作用于He La和Si Ha细胞不同时间后,采用MTT法观察药物对细胞活力的影响,流式细胞仪检测凋亡率,划痕试验观察细胞迁移率,基质胶法测定细胞粘附百分率,Transwell试验检测侵袭细胞数,Western blotting分析CAT对Si Ha细胞抑制转移蛋白nm23-H1和促转移蛋白MTA1表达的影响。结果 CAT显著抑制He La和Si Ha细胞活力,增加细胞凋亡率;降低细胞迁移百分率和黏附百分率;减少侵袭细胞数;明显增加Si Ha细胞nm23-H1蛋白表达,降低MTA1蛋白表达。结论 CAT显著诱导人宫颈癌He La和Si Ha细胞凋亡,抑制其侵袭迁移,提示其具有潜在的抗宫颈癌作用。

Objective To examine whether casticin influences the apoptosis, migration, and invasion of human cervical cancer HeLa and SiHa cells. Methods HeLa and SiHa cells were treated with various concentration of casticin, cell viability was examined by MTT assay and the apoptosis induced by casticin was determined by flow cytometry. The scratch test, matrigel assay, and Transwell test were performed respectively to estimate the capabilities of migration, adhesion, and invasion of HeLa and SiHa ceils. The protein expression of nm23-H1 and MTA1 in SiHa cells was analyzed by Western blotting. Results Casticin treatment significantly inhibited cell viability, increased apoptosis rate, decreased migration percentages and adhesion of HeLa and SiHa cells, and reduced invasion cells number. The protein expression of nm23-H1 was evidently enhanced, but that of MTA 1 was significantly decreased. Conclusion Casticin markedly induces the apoptosis and suppresses the migration and invasion of HeLa and SiHa cells, suggesting its potential therapeutic effects on cervical cancer.