炎调方对脓毒症急性肺损伤大鼠肺组织髓过氧化物酶和丙二醛水平的影响

Influence of Yantiaofang on myeloperoxidase and malondialdehyde in lung tissue of rats with acute lung injury induced by sepsis

目的观察炎调方对脓毒症急性肺损伤(ALI)大鼠肺组织髓过氧化物酶(MPO)、丙二醛(MDA)水平的影响。方法清洁级健康雄性SD大鼠随机分为对照组、假手术组、模型组、炎调方组、地塞米松组,炎调方组ig 9.9 g/kg炎调方,地塞米松组ig 0.45 mg/kg地塞米松,每天给药1次,连续给药3 d。末次ig 2 h后采用盲肠结扎穿孔术(CLP)制备脓毒症ALI模型,分别于造模后24 h处死动物,进行肺组织HE染色,测定各组大鼠湿/干重比(W/D),肺组织MPO、MDA水平。结果模型组大鼠肺组织损伤程度、MPO及MDA水平较对照组及假手术组显著增高(P<0.01),炎调方组、地塞米松组肺组织损伤程度、MPO及MDA水平较模型组显著降低(P<0.05、0.01)。结论炎调方可有效减轻脓毒症ALI大鼠的炎症及氧化应激反应。

Objective To observe the influence of Yantiaofang on myeloperoxidase （MPO） and malondialdebyde （MDA） in lung tissue of rats with acute lung injury induced by sepsis. Methods Male clean SD rats were randomly divided into control group, Sham group, model group, Yantiaofang group, and dexamethasone group. Rats of Yantiaofang group and dexamethasone group were fed respectively by 9.9 g/kg and 0.45 mg/kg dosage once daily for 3 d. After 2 h of the last administration, acute lung injury induced by sepsis model was reproduced in rats with cecal ligation and puncture （CLP）. Each rat was tested after the 24 h when the CLP completed. Histopathological sections of the lung tissues of rats were stained by HE for the observation of histopathologieal changes. The ratio of W/D and the contents of MPO and MDA in lung tissue were also measured for the observation of inflammation. Results Compared with control group and Sham operation group, there was significant increase in the ratio of W/D, MPO and MDA in rat lung tissue of model group （P 〈 0.01）. On the other side, compared with model group, the ratio of W/D, MPO and MDA in rat lung tissue of Yantiaofang group and dexamethasone group were decreased （P 〈 0.05, 0.01）. Conclusion Yantiaofang can effectively decrease the inflammation and oxidative stress in rats with acute lung injury induced by sepsis.