板蓝根多糖对环磷酰胺造模大鼠的双向免疫调节作用

Dual-directional immune regulation of Isatidis Radix polysaccharides on rats treated with cytoxan

目的研究板蓝根多糖（IRPS）对机体不同免疫状态的调节作用。方法 36只颈静脉插管大鼠,随机分为6组：免疫正常（NS）组、免疫亢进（IH）组、免疫抑制（IS）组、IRPS组、IH＋IRPS组、IS＋IRPS组,每组6只;各组大鼠均ip鸡卵清蛋白进行免疫,应用环磷酰胺（Cy）注射制备大鼠IH和IS模型,ig 60 mg/kg IRPS,每天给药1次,连续给药3 d;免疫后第6和12天,每组大鼠处死3只,测定T、B淋巴细胞增殖情况和NK细胞活性,试剂盒法检测各组血清OVA抗体水平;免疫后第1～10、12天,颈静脉采血,分离血清,液相芯片技术检测γ-干扰素（IFN-γ）、白细胞介素-2（IL-2）、白细胞介素-4（IL-4）、白细胞介素-6（IL-6）、白细胞介素-10（IL-10）以及肿瘤坏死因子-α（TNF-α）水平。结果 IRPS对于不同免疫状态大鼠的B淋巴细胞增殖、NK细胞活性均具有双向调节作用;对Th1型细胞因子IFN-γ、TNF-α和Th2型细胞因子IL-4、IL-6、IL-10具有双向调节作用;对免疫功能正常大鼠具有免疫增强作用。结论 IRPS能够抑制IH组大鼠的亢进趋势,提升IS组大鼠的免疫功能,对Cy造模大鼠具有双向免疫调节作用。

Objective To investigate the regulation of lsatidis Radix polysaccharides （IRPS） to body in different immune status. Methods Totally 36 rats fixed with juglar vein catheter were randomly divided into six groups： normal group （NS）, immune hyperfunction （IH） group, immunosuppressive （IS） group, IRPS group, IH ＋ IRPS group, and IS ＋ IRPS group, six rats in each group. The immune hyperfunctional and immunosuppressed model rats were established by Cytoxan injection, and treated with 60 mg/kg IRPS once daily for 3 d. After 6 and 12 d of immunization, three rats were killed. Lymphocyte proliferation and NK cell activity were determined. A kit was used to detect the level of serum OVA antibody. After 1-10 and 12 d of immunization, collected jugular vein blood, separated serum, and liquid chip technology was used to detect the levels of IFN-γ, IL-4, IL-2, IL-6, IL-10, and TNF-α. Results The proliferation ability of B lymphocytes and NK cells activity in both IH and IS groups were regulated, and the secretion of Thl cytokines IFN-γ, TNF-α and Th2 cytokines IL-4, IL-6, and IL-10 was dual-directional regulated by IRPS. The immune function of control group was enhanced by IRPS. Conclusion It is indicated that IRPS can inhibit the accentuation of immune system in hyperfunction group, and enhance the immune function ofimmunosuppressed rats, so we conclude that the IRPS has a dual regulation effect on immune system of model rats treated with cytoxan.