中国南海豆荚软珊瑚Lobophytum sp.中西松烷二萜类化学成分研究

α-Methylene-γ-lactone-bearing Cembranoid Diterpenes from the South China Sea Soft Coral Lobophytum sp.

为了从中国南海生物中寻找具有生物活性的次级代谢产物,本研究选择采自三亚附近海域的一种豆荚软珊瑚Lobophytumsp.为研究对象,综合运用各种色谱分离技术和现代光谱学方法,对其甲醇提取物中的西松烷二萜类化学成分进行了分离鉴定,得到7个α-亚甲基-γ-内酯型西松烷二萜类化合物：Lobophytolide B（1）,3-dehydroxyl-20-acetylpresinularolide B（2）,crassumolide I（3）,lobophilide B（4）,durumolide C（5）,lobolide（6）,13-hydroxylobolid（7）。采用SRB或MTT法对化合物2-7进行了肿瘤细胞毒活性初步评价,活性测试结果显示这些化合物均表现出不同程度的肿瘤细胞毒活性。

In order to search for bioactive metabolites from marine invertebrates, a South China Sea soft coral Lobophyturn sp. was studied for cembranoid components. Chromatography techniques and spectro- scopic methods were used to isolate and identify the compounds. And seven e-methylene-7-1actone-bearing cembranoid diterpenes were obtained as lobophytolide B （1）, 3-dehydroxyl-20-acetylpresinularolide B （2）, crassumolide I （3）, lobophilide B （4）, durumolide C （5）, lobolide （6） and 13-hydroxylobolid （7）, respec- tively. Compounds 2-7 were evaluated for their cytotoxicity against HeLa and P388 tumor cell lines.