胰腺癌组织TPAP基因启动子区甲基化的临床意义

Clinical significance of the methylation in TPAP promoter in pancreatic cancer

目的探讨TPAP基因甲基化在胰腺癌发生和发展中的作用及临床意义。方法收集2006年7月至2009年8月上海长海医院经病理确诊的68例胰腺癌患者手术切除的胰腺组织，采用甲基化特异性PCR检测癌组织以及癌旁正常胰腺组织中TPAP基因启动子区甲基化状态。结果胰腺癌组、癌旁正常胰腺组TPAP基因启动子区甲基化率分别为（0．214±0．057）％和（0．084±0．096）％，胰腺癌的甲基化率显著高于癌旁正常胰腺，差异有统计学意义（P=0．003）。TPAP基因启动子区甲基化率与胰腺癌患者年龄、性别、肿瘤分化程度、淋巴转移及CEA、CA19—9水平均无相关性，而与远处转移呈正相关。结论TPAP基因可能参与胰腺癌侵袭转移，TPAP基因启动子区高甲基化与胰腺癌的发生、发展密切相关。

Objective To investigate functional role and clinical significance of the methylation in the promoter of TPAP gene in the development and progression of pancreatic cancer. Methods Surgically resected specimen from 68 patients who were pathologically diagnosed as pancreatic cancer in Changhai Hospital from July 2006 to August 2009 were collected. The methylation in the promoter of TPAP gene in tumor and non- tumor adjacent tissue was detected by methylation specific PCR. Results The methylation rate of tumor and non-tumor adjacent tissue was （ 0. 214 ＋ 0. 057 ） % and （ 0. 084 -＋ 0. 096 ） % , respectively, and pancreatic cancer tissue had significantly higher methylation rate than the adjacent tissue. Hypermethylation of TPAP gene was not correlated with age, gender, tumor differentiation, lymphatic metastasis, serum CEA and CA19-9, but was positively correlated with distant metastasis. Conclusions Hypermethylation in the promoter of TPAP gene may participate in the invasion and metastasis of pancreatic cancer and the hypermethylation of the promoter is closely associated with the tumorigenesis and development of pancreatic cancer.