缺氧诱导因子-1α基因G1790A或导致COPD易感性

HIF-1α polymorphisms G1790A may lead to susceptibility to chronic obstructive pulmonary disease

目的：探讨HIF-1α基因多态性于与COPD易感性之间的关系。方法：利用RT-PCR、Western blot、ELISA检测COPD病例组和对照组（各16例）人群肺组织标本中HIF-1a mRNA及HIF-1a蛋白的表达水平。利用PCR-RFLP检测COPD病例组（120例）及健康对照组（112例）人群HIF-1α基因C1772T和G1790A的多态性分布；结果：RT-PCR结果显示HIF-1a mRNA在对照组和病例组中表达无差异（P＞0.05）；Western blot和ELISA检测结果示对照组HIF-1α蛋白表达高于病例组（P0.05），G1790A基因型频率分布有差异（P〈0.05）。结论：HIF-1α基因G1790A单核苷酸多态性或导致COPD病人易感，

Purpose： To explore whether hypoxia-inducible factor-1α （HIF-1α） polymorphisms are associated with susceptibility to chronic obstructive pulmonary disease （COPD）. Materials and Methods： Sixteen patients with and sixteen healthy patients were conducted with Real time PCR、Western blot、ELISA to test the HIF-1α mRNAand HIF-1a protein levels.One hundred twenty patients with COPD and 112 healthy controls were recruited from the Han population in Southern China. PCR-RFLP was used to assess C1772T and G1790A polymorphisms of the HIF-1α gene, and differences of genotypes between the two groups were compared. Results： RT-PCR results show that HIF-1a mRNA level of control group is higher than in the group of COPD, with a t-test p〉0.05. Western blot and ELISA results indicated the hIF-1α protein level is higher in control group than in COPD group ,with a P0.05）. In contrast, distributions of G1790A polymorphisms were significantly different between the two groups （P〈0.05）.