糖原合成酶激酶3β对外泌体蛋白质和RNA产量的影响

Effect of Haiying J iaotong GSK3β knockdown on human mast cell exosome protein and RNA profiles

目的：多种细胞均可以释放外泌体，但外泌体复杂的释放机制尚未完全阐明。糖原合成激酶3β是多种细胞反应的关键因子，本文旨在阐明其对肥大细胞释放外泌体蛋白质和RNA的影响。方法：敲除肥大细胞的GSK3β蛋白，通过差速离心的方法获取外泌体。利用生物分析仪和western blot分别对外泌体总RNA和总蛋白含量进行比较分析。结果：敲除GSK3β蛋白肥大细胞的外泌体中蛋白质和RNA与对照组相比含量升高。结论：糖原合成激酶3β在肥大细胞外泌体释放中具有重要的作用，且其影响了外泌体中蛋白质和RNA的释放量。

Objective： Exosomes are vesicles of endocytic original released by many cells. The mechanism of exosome released is very complicated and unclear. Glycogen Synthase Kinase 3β is a key factor for diverse cellular response. However, the effect of GSK3β knockdown on human mast cell exosome release and RNA profiles is still unknown. Methods： In this study, we use the cells, which were knockdown of GSK3β protein following transduction with GSK3β-targeted shRNA. The total RNA of exosomes were analyzed by Bioanalyzer and proteins were analyzed by western blot. Results： Our results show that the protein and RNA of exosome release from human mast cells with GSK3β knockdown are increased compared with the scramble RNA transducted HMC-1 cells. Conclusions： Glycogen Synthase Kinase 3β plays a role in the release of exosome from human mast cells. Furthermore, the GSK3β have an effect in the protein and RNA of exosome released.