地鳖多糖提取物的体内外抗氧化作用

Antioxidant effects of polysaccharide extraction from Eupolyphaga sinensis Walker( ESW) in vitro and in vivo

目的研究地鳖多糖提取物体内外抗氧化作用。方法体外试验采用分光光度法、邻苯三酚自氧化法和Fe2+-邻二氮菲法分别检测地鳖多糖对二苯代苦味酰基自由基(DPPH·)、超氧阴离子自由基(O-2·)和羟自由基(·OH)的清除,检测体外红细胞氧化溶血和肝线粒体肿胀程度。体内试验小鼠连续灌胃不同剂量(0、40、80、160 mg/kg)地鳖多糖提取物20 d,硫代巴比妥酸(TBA)比色法测定小鼠组织中丙二醛(MDA)含量,分光光度法检测抗氧化酶(SOD、CAT、GSH-Px)活力。结果与对照组比较,地鳖多糖组对自由基清除率随多糖浓度升高而显著提高;体外红细胞氧化溶血和线粒体肿胀显著降低。多糖组小鼠肝肾中MDA含量降低,SOD、CAT、GSH-Px抗氧化酶活力提高并明显高于对照组。结论地鳖多糖提取物能够清除自由基,降低自由基引起细胞氧化损伤,抑制组织中过氧化物生成,提高抗氧化酶活力,具有显著的体内外抗氧化作用。

Objective To investigate the antioxidant effects of polysaccharide extraction from Eupolyphaga sinensis Walker（ ESW） in vitro and in vivo. Methods The methods of spectrophotometry,pyrogallol autoxidation and Fe2 ＋phenanthroline were used to detect the free radicals scavenging（ DPPH·,O-2·and·OH）,erythrocyte oxidative hemolysis and liver mitochondrial swelling of polysaccharide extraction from ESW in vitro. The mice were administered with different doses of polysaccharide extraction from ESW（ 0,40,80,160 mg / kg） for 20 days in vivo. The contents of MDA in different tissues of the mice were determined by TBA method,and spectrophotometry was used to assay the activity of antioxidant enzymes（ SOD,CAT,GSH-Px）. Results Compared with the control group,free radical（ DPPH·,O-2· and ·OH）scavenging rate was significantly improved with the increasing concentration of polysaccharide extraction. There was a significant decrease in the oxidation hemolysis of erythrocytes and mitochondrial swelling in vitro. MDA contents were decreased in the polysaccharide group,and the activities of SOD,CAT and GSH-Px were significantly higher than those in the control group. Conclusions Polysaccharide extraction from ESW can eliminate free radicals,reduce cell oxidative damage caused by free radicals,inhibit the formation of peroxide in tissues,and increase the activity of antioxidant enzymes,indicating a good antioxidant property.