碳源对多粘类芽孢杆菌生长和多粘菌素E合成的影响

Effect of carbon sources on growth of Paenibacillus polymyxa and polymyxin E synthesis

为探究提高多粘菌素E产量的方法,在发酵培养基中分别添加葡萄糖、蔗糖、麦芽糖、乳糖和可溶性淀粉,应用平板菌落计数和HPLC法研究了其对多粘类芽孢杆菌生长和多粘菌素E产量的影响。结果表明,5种碳源对多粘类芽孢杆菌的生长和多粘菌素E产量的影响不同,可溶性淀粉是菌体生长的最适碳源,且多粘菌素E产量依次为可溶性淀粉>乳糖>蔗糖>麦芽糖>葡萄糖。可溶性淀粉有助于延长多粘菌素E的产素期和提高其合成速率,与葡萄糖作碳源相比,多粘菌素E的产量和合成速率分别提高了111%和60%。因此,可溶性淀粉是多粘菌素E合成的最适碳源。

In order to explore newmethods to improve the yield of polymy^xin E,five different carbon sources including glucose,sucrose, maltose, lactose and soluble starch were added in the fermentation medium,respectively.The influence on Paenibacillus polymyxa growth and polymyxin E production of different carbon sources were investi-gated through plate counting method and HPLC method. The results indicated that the effects of different carbon sources on the growth of Paenibacillus polymyxa and the polymyxin E production varied significantly. The effect of these carbon sources on polymyxin E production was： soluble starch 〉 lactose 〉 sucrose 〉 maltose 〉 glucose. Soluble starch promoted both cell growth and polymyxin E yield by extending the polymyxin E biosynthetic period and promo-ting the synthetic rate. Compared with glucose as carbon source,the yield and the synthetic rate of polymyxin E were increased by 111% and 60% ,respectively. Therefore,soluble starch was the optimal candidate for carbon sources among the five carbohydrates in polymyxin E synthese.