摘要
目的对复方氯甲霜的质量标准进行再提高研究。方法色谱柱为Agi Lent XDB-C_(18)(150 mm×4.6 mm,5μm);流速为1 m L·min^(-1);柱温为30℃;进样量为10μL;氯霉素的流动相为甲醇-庚烷磺酸钠缓冲溶液(32∶68);紫外检测波长为277nm;甲硝唑的流动相为甲醇-水(20∶80);紫外检测波长为320 nm。结果氯霉素、甲硝唑进样量分别在5.56~111.2,26.02~260.2 mg·L^(-1)浓度范围内与峰面积呈良好的线性关系,r分别为1.000 0和0.999 6(n=6);平均回收率分别为103.8%,99.6%,RSD分别为1.73%和0.43%(n=9)。结论该方法简便快速、准确、重复性好,可用于该制剂中氯霉素及甲硝唑的含量测定。
Objective To improve quality standards of Chloramphenicol Metronidazole Cream. Methods Chloramphenicol andmetronidazole were assayed by HPLC. Agi Lent XDB-C18( 150 mm × 4. 6 mm,5 μm) was used at the rate of 1. 0 m L·min^-1. The injection volume was 10 μL and the column temperature was set at 30 ℃. 277 nm and 320 nm were selected asthe detection wavelengths forchloramphenicol and metronidazole respectively,and themobile phasesweremethanol-sodiumheptanesulfonate( 32∶ 68) forchloramphenicol and methanol-water( 20 ∶ 80) formetronidazole,respectively. Results A good linearrelationship was established between the peak response and the concentration of chloramphenicol and metronidazole over the range of 5. 56 - 111. 2 mg·L^-1( r = 1. 000 0) and 26. 02- 260. 2 mg· L^-1( r = 0. 999 6) respectively. The mean recoveries of chloramphenicol and metronidazole were103. 8 %( RSD =1. 73%) and 99. 6 %( RSD = 0. 43%),respectively. Conclusion The method is simple,accurate and repeatable,which can be used for the determination of Chloramphenicol Metronidazole Cream.
出处
《安徽医药》
CAS
2016年第7期1273-1275,共3页
Anhui Medical and Pharmaceutical Journal
关键词
色谱法
高压液相
氯霉素
甲硝唑
Chromatography
High Pressure Liquid
Chloramphenicol
Metronidazole