高效液相色谱法测定消炎止痢灵片中甲氧苄啶的含量

Determination of trimethoprim in Xiaoyanzhililing pian by HPLC

目的建立测定消炎止痢灵片中甲氧苄啶含量的高效液相色谱法。方法采用高效液相色谱法,色谱柱为Phenomenex Luna C_(18)(5μm,4.6 mm×250 mm),流动相为乙腈-0.5%磷酸溶液(含1 mmol·L^(-1)醋酸铵)(33∶67),检测波长为270 nm。结果甲氧苄啶在0.1002~3.002μg范围内与峰面积值呈良好的线性关系(相关系数为0.999 9);平均回收率(n=6)为99.59%,RSD为0.22%。结论该方法简单,准确性高,精密度好,适合消炎止痢灵片中甲氧苄啶的含量测定。

Objective To establish a content determination method of trimethoprim in Xiaoyanzhililing pian by HPLC. Method Determined by HPLC,the column was Phenomenex Luna C18（ 5 μm 4. 6 mm × 250 mm）,the mobile phase was acetonitrile- 0. 5% Phosphoric acid（ include 1 mmol·L^-1 ammonium acetate）,the ditection wavelength was 270 nm. Result Trimethoprim was linear in the range of 0. 1002 - 3. 002 μg（ r = 0. 999 9）; the average recovery was 99. 59% and RSD was 0. 22%（ n = 6）. Conclusions This method is simple,accurate,precision and available for the determination trimethoprim in Xiaoyanzhililing pian.