PM2.5暴露对鸦胆子油乳治疗荷瘤肺癌小鼠腹腔巨噬细胞的作用

Effect of PM2.5 exposure for peritoneal macrophages of tumor-bearing mice with lung cancer treated by bruceolic oil emulsion

目的探讨PM2.5暴露对鸦胆子油乳治疗荷瘤肺癌小鼠腹腔巨噬细胞的活性调节作用。方法建立肺癌荷瘤A549细胞小鼠模型32只,随机分为4组:治疗组1(鸦胆子油乳治疗组)和对照组1,治疗组2(PM2.5暴露联合鸦胆子油乳治疗组)和对照组2,每组8只。收集腹腔巨噬细胞,检测520 nm吸光度值。结果腹腔巨噬细胞吞噬能力显示,治疗组1高于对照组1,差异有统计学意义(P<0.05);治疗组2低于对照组2及治疗组1高于治疗组2,差异有统计学意义(P<0.01)。结论 PM2.5暴露对鸦胆子油乳治疗荷瘤肺癌小鼠腹腔巨噬细胞的活性有抑制作用。

Objective To explore active regulating effect of PM2.5 exposure for peritoneal macrophages of tumor-bearing mice with lung cancer treated by bruceolic oil emulsion. Methods 32 mouse models in tumor-bearing A549 cell in lung cancer were randomly divided into 4 groups:treatment group 1(bruceolic oil emulsion treatment group) and control group 1,treatment group 2(PM2.5 exposure combined with bruceolic oil emulsion treatment group) and control group 2.Each group had eight mice.Peritoneal macrophages from these mice were collected and 520 nm absorbance degree value was detected. Results phagocytic ability of peritoneal macrophage indicated that treatment group 1 was higher than that of control group 1,and there was a statistical difference(P<0.05).The phagocytic ability in the treatment group 2was lower than that of control group 2,and the phagocytic ability in the treatment group 1 was higher than that of treatment group 2, and there was a statistical difference(P<0.01). Conclusion PM2.5 exposure has inhibiting effect for activity of peritoneal macrophages in tumor-bearing mice with lung cancer treated by bruceolic oil emulsion.