市政剩余污泥蛋白质碱提取资源化利用工艺研究

Resource utilization of protein alkali extraction from municipal residual sewage sludge

研究了市政剩余污泥的蛋白质提取工艺。首先比较了酸碱两种预处理方法对蛋白质提取的影响,对效果较好的碱处理方法进行了进一步优化。利用响应面法对NaOH浓度、溶液加入量和处理时间进行了优化,获得了最优蛋白质提取条件:21.79 g湿污泥中加入80 m L NaOH溶液(3.68 mol/L),在50℃条件下处理20.32 h。在上述条件下,蛋白质产量为8 967±0.4μg/g干污泥,是优化前的2.33倍。

We address protein extraction process of municipal residual sewage sludge. We initially compared acid and alkaline hydrolysis pretreatment methods and optimized the better alkaline hydrolysis method for protein extraction 什 om municipal residual sludge. To acquire optimal extraction condition, we optimized NaOH concentration, solution amount and pretreatment time with response surface method. The optimal protein extraction condition is 21. 79 g wet municipal sludge, 80 mL NaOH solution （3.68 mol/L）,and pretreatment time of 20. 32 h at 50 ℃. The optimal protein extraction yield is 8 967 ± 0.4 ug/g dry sludge, 2.33 times of initial extraction yield.