Rif1通过BMP4信号传导通路影响小鼠胚胎干细胞自我更新和分化的作用机制

Effects and mechanisms of Rif1 on mouse embryonic stem cell self-renewal and differentiation through BMP4 signal transduction pathway

[目的]研究Rif1通过BMP4信号传导通路影响小鼠胚胎干细胞自我更新和分化的作用机制.[方法]从基因表达数据库中下载基因芯片数据(GSE 55129),采用R软件包分别对长期、短期干扰Rif1后的表达谱数据做预处理并筛选差异基因.取长期干扰Rif1的差异基因进行KEGG分析,采用RT-qPCR对KEGG结果进行验证,取短期干扰Rif1的差异基因进行GO分析,选择与胚胎发育有关的基因整合到相关的KEGG通路中.[结果]KEGG分析结果显示,Rif1与TGF-β(BMP4)信号传导通路高度相关(P<0.01);RT-qPCR验证结果证实BMP4表达明显升高(P<0.05);GO分析结果与活化BMP4通路后的效应相一致.[结论]Rif1通过BMP4信号传导通路影响小鼠胚胎干细胞的自我更新和分化.

OBJECTIVE To investigate the mechanisms of Rifl on mouse embryonic stem cell self-renewal and differentiation through BMP4 signal transduction pathway. METHODS The expression data of gene microarray GSE55129 were downloaded from Gene Expression Omnibus. R software package was used to preprocessed the gene expression data after the long term and short term interference of Rifl, and differentially expressed genes（DEGs） were screened. DEGs of long term interference of Rifl were analyzed using KEGG, and DEGs of short term interference of Rifl were analyzed using GO analysis. Furthermore, RT-qPCR was used to verify the results of KEGG, Then the genes related to embryonic development of GO analysis were integrated to relevant KEGG pathway. RESULTS The results of KFGG showed that Rifl was highly correlated to the TGF-β（BMP4） signaling pathways（P〈0.01）. The results of RT-qPCR verified the high expression of BMP4（P〈0.05）, and the results of GO analysis were in line with the effects of BMP4 pathway activation. CONCLUSION Rifl affected mouse embryonic stem cell self-renewal and differentiation through BMP4 signal transduction pathway.