脾虚证多发性抽动症小鼠模型的建立及评价

Establishment and preliminary evaluation of a mouse model of tourette's syndrome(TS) with spleen deficiency syndrome

目的建立脾虚证多发性抽动症小鼠动物模型,初步了解该脾虚模型的一般特征。方法腹腔注射亚氨基二丙腈350 mg/(kg·d)结合浓度为1 g/m L的番泻叶颗粒剂溶液20 m L/(kg·d)灌胃,连续8天,建立脾虚证多发性抽动症小鼠模型。通过自主活动计数、刻板运动评分、体质量、食量、大便粒数、胸腺及脾脏指数、胃泌素、D-木糖等指标对脾虚证多发性抽动症小鼠进行评价。结果模型组、脾虚组小鼠造模后在相同的时间内活动路程及平均速度较空白对照组明显增加(P<0.05),体质量增加缓慢。脾虚组小鼠的食量降低,但差异无统计学意义(P>0.05)。胸腺指数、脾脏指数及胃泌素、D-木糖浓度均无统计学差异(P>0.05)。结论本模型抽动症状稳定,具有脾虚证的某些症状,一定程度上具备了脾虚证多发性抽动症的表面效度。

Objective To establish an animal model of TS in mouse with spleen deficiency syndrome,and to explore the general characteristics of the model of spleen deficiency. Methods Mice were intraperitoneal injected iminodipropionitrile 350 mg /（ kg · d） and intragastric administration Senna granules solution 20 m L /（ kg·d） for 8 days. The independent activity counts,stereotyped movement score,body weight,food intake,feces granu,thymus and spleen index,gastrin,D-xylose and other indexes were used to evaluate TS mouse of spleen deficiency. Results After modeling,the activity distance and average speed of model group and spleen deficiency group was significantly increased,and body weight increase slowly. Food intake of spleen deficiency group was decreased（ P 〉0. 05）. Thymus index,spleen index,gastrin and D-xylose concentration had no significant difference. Conclusion The TS model has stable symptoms and some symptoms of spleen deficiency syndrome,and it has the face validity of the syndrome of deficiency of the spleen.