摘要
目的探讨定量测定血清高尔基体蛋白73(GP73)的临床意义。方法采用双抗体夹心酶联免疫吸附法(ELISA)测定肝癌治疗前、肝癌介入术后、肝癌切除术后、肝移植术后、肝癌复发、乙肝肝硬化、慢性乙型肝炎、乙肝携带者、慢性丙型肝炎、脂肪肝以及健康体检血清标本中的GP73浓度。结果肝癌治疗前病例组中GP73的ROC曲线下面积为0.983,敏感性和特异性分别为96.8%和93.9%;对比肝癌治疗前,介入术后GP73浓度下降(P<0.001),肝癌切除和肝移植术后GP73浓度下降(P<0.001),肝癌复发伴随GP73浓度升高。与健康体检组比较,GP73浓度在肝硬化病例组中显著升高(P<0.001),但是低于肝癌治疗前病例组中GP73浓度(P<0.001)。在慢性乙型肝炎、乙肝携带者、脂肪肝病例组中均可检测到GP73,但显著低于肝癌治疗前病例组(P<0.001)。GP73浓度与肝癌分期的相关性不明显。6种非肝脏恶性肿瘤标本中GP73浓度显著低于未治疗的肝癌标本(P<0.001)。GP73在丙肝相关肝病的浓度均显著高于乙肝相关肝病(P<0.001)。结论 GP73可作为辅助临床早期诊断肝癌的一种标志物,对肝癌术后的复发及肝硬化的进展也有监测作用。
Objective To study the diagnosis significance of serum Golgi protein 73(GP73) in patients with hepato- cellular carcinoma(HCC). Methods The double antibody sandwich EL1SA quantitative measurement methods were used for the detection of serum GP73 level in a total of 6 069 subjects, including patients with HCC before surgery, patients with HCC after intervention, hepatectomy, transplantation or recurrence, patients with cirrhosis, patients with chronic hep- atitis B, HBV carriers, patients with chronic hepatitis C, patients with fatty liver and healthy adults. Results The AUROC for GP73 was 0.983, the sensitivity was 96.8% and specificity was 93.9% in patients with HCC before surgery. GP73 lev- els in patients with HCC decreased following intervention, surgical resection or transplantation and increased with tumor recurrence (P 〈 0.001). In contrast, the median value of GP73 in patients with cirrhosis was significantly higher than that of healthy subjects (P 〈 0.001) but lower than that of HCC subjects (P 〈 0.001). GP73 could be detected in patients with chronic hepatitis B, HBV carriers and patients with fatty liver, which was significantly lower than that of HCC subjects (P 〈 0.001). The correlation between GP73 level and staging of HCC was not remarkable. The level of GP73 was signifi- cantly higher in HCC than the other kinds of tumors(P 〈 0.001). GP73 levels in hepatitis C patients were significantly higher than those of patients with hepatitis B (P 〈 0.001). Conclusion GP73 is an accurate serum marker for the early detection of HCC and it increases after surgery and may be also used in the surveillance of cirrhosis.
出处
《北京医学》
CAS
2016年第9期894-899,共6页
Beijing Medical Journal
关键词
高尔基体蛋白73
甲胎蛋白
肿瘤标志物
肝癌
Golgi protein 73(GP73)
alpha fetoprotein(AFP)
tumor marker
hepatocellular carcinoma(HCC)