镇江市2012年-2015年HIV抗体不确定者检测结果分析

Analysis of indeterminate results of HIV antibodies in Zhenjiang during 2012-2015

目的分析免疫印迹试验(WB)"HIV抗体不确定"结果特征及跟踪监测结果,为尽早明确个体诊断提供科学依据。方法回顾性收集本市2012年-2015年共16例HIV抗体不确定者,分析人群分布特点、ELISA吸光度与临界值(S/CO)的比值分布特点、不确定结果带型分布特点、受检者随访复检情况及最终抗体转归情况。结果本市16例样本主要来源于医院就诊患者,占56.25%,其次为男男性行为者(25.00%)及无偿献血者(18.75%)。带型分布以gp160/p24为主,占75.00%。经随访,1例判定为阴性,11例抗体阳转,共4例失访。结论在WB不确定结果中如同时出现gp160/p24,且经流行病学调查存在HIV感染高危因素,应高度怀疑HIV感染,需加强随访,并尽快开展辅助诊断。

Objective To analyze the characteristics of the indeterminate results of HIV antibody with Western Blot and track monitoring results,so as to provide scientific basis for the early diagnosis.Methods A total of 16 cases with the indeterminate HIV antibody results were retrospectively collected from Zhenjiang druing 2012-2015,so as to analyze the distribution characteristics of the 16 cases,the proportion of optical density value to cut-off value（S/CO） of ELISA,the bands distribution of indeterminate results,the retest of medical follow-up and the final HIV antibody outcome.Results 16 cases,accounting for56.25%,were from hospital patients,followed by the population of men who have sex with men（25.00%） and the blood donors（18.75%） respectively.The main bands of Western Blot was gp160/p24,accounted for 75.00%.With following-up,11 cases were positive and 1 case was negative,with 4 cases lost.Conclusion If WB indeterminate results happened with the gp160/p24,and the risk factors of HIV infection existed by epidemiological investigation,which should be highly suspected as HIV infection,so we need to strengthen follow-up,and to carry out the auxiliary diagnosis as soon as possible.