重症急性胰腺炎患者血清IL-17、IL-17R、IL-6、HMGB1和IL-10水平变化及临床意义

Levels changes and its clinical significance of serum IL-17,IL-17R,IL-6,HMGB1 and IL-10 in patients with severe acute pancreatitis

目的探讨重症急性胰腺炎(SAP)患者血清IL-17、IL-17R、IL-6、HMGB1及IL-10水平的变化和临床意义。方法研究对象包括29例正常对照患者和54例重症急性胰腺炎患者,患者组根据是否并发多器官功能障碍综合征(MODS)分为MODS组和非MODS组。采用ELISA试剂盒检测HMGB1、IL-17、IL-17R、IL-6、IL-10水平。结果SAP患者IL-17、IL-17R、IL-6水平均高于正常对照组,而IL-10低于正常对照组,差异均具有统计学意义(P<0.05)。正常对照组HMGB1为阴性,SAP患者显著升高。MODS组IL-6、IL-17、IL-17R、HMGB1水平高于非MODS组,而IL-10低于非MODS组,差异均有统计学意义(P<0.05)。结论重症急性胰腺炎患者血清促炎细胞因子升高,抑炎细胞因子降低,晚期炎症介质显著升高,可能存在炎症细胞因子网络失衡,与疾病的严重程度相关。

Objective To investigate the level changes and its clinical significance of serum IL-17,IL-17 R,IL-6,HMGB1 and IL-10 in patients with severe acute pancreatitis（SAP）.Methods Totally 54 SAP patients were divided into MODS group and non-MODS group according to MODS condition while admission was included in this study,and 29 healthy volunteers served as normal controls.The serum levels of IL-17,IL-17 R,IL-6,HMGB1 and IL-10 were measured by ELISA on admission.Results The serum IL-17,IL-17 R,IL-6 levels were markedly elevated in SAP patients than those in normal controls,while the serum IL-10 levels were markedly reduced than those in normal controls,with the differences statistically significant（P〈0.05）.HMGB1 in the normal control group was negative and increased significantly in SAP patients.The serum IL-6,IL-17,IL-17 R,HMGB1 in MODS patients were significantly higher than those without MODS on admission,while IL-10 was lower than the non-MODS group,with the differences statistically significant（P〈0.05）.Conclusion The elevated serum levels of proinflammatory cytokines and reduced serum levels of anti-inflammatory cytokines exist in severe acute pancreatitis patients with a significant rise of inflammatory mediators in late stage.There may be an inflammatory cytokine network imbalance in SAP,which may be associated with the severity of the disease.