ERCC1 mRNA及P53表达对局部晚期宫颈癌治疗决策的影响及关联性研究

Studying roles and relation of expression of ERCC1 mRNA and P53 genein to locally advanced cervical cancer neoadjuvant chemothearapy

目的探讨局部晚期宫颈癌（LACC）组织中ERCC1 mRNA及P53蛋白表达与新辅助化疗（NACT）敏感性及预后的关系。方法采集60例病例,在NACT前分别应用RT—PCR（逆转录聚合酶链反应）技术及免疫组化SP方法检测ERCC1mRNA和P53蛋白的表达。结果 60例患者中,有效45例,有效率为75.00%。患者年龄、病理分级及类型与NACT疗效差异均无统计学意义（P﹥0.05）。局部晚期宫颈癌组织中ERCC1mRNA、P53蛋白的表达与NACT疗效有关（P〈0.05）。其中ERCC1mRNA及P53过表达均为NACT疗效的危险因素,分别[β=-1.398OR（95%CI）=0.247（0.069-0.880）],[β=-1.981,OR（95%CI）=1.375（0.903-1.519）]。术后病理结果提示具有放疗高危因素者共31例（68.9%）。二者过表达均可提示术后病理结果具有放疗高危因素（P〈0.05）。且二者表达水平呈显著正相关（r=0.275,P〈0.05）。结论 LACC组织中ERCC1mRNA及P53表达与NACT敏感性相关,并且可提示术后病理结果具有放疗高危因素,两者表达呈正相关。

Objective To investigate association between expression of ERCC1 mRNA and P53 gene with the neoadjuvant chemotherapy （NACT） response and prognosis of locally advanced cervical cancer （LACC）. Methods A total of 60 biopsies from LACC patients were collected before chemotherapy in Henan Cancer Hospital. Using these paraffin embedded samples, the expression of ERCC1 mRNA was detected RT - PCR technique,and the expression of P53 protein was examined by immunohistochemistry （ IHC ）. Results Our results indicate that 45 out of 60 patients demonstrated positive response to NACT, and the effective rate was 75 %. In addition, The logistic regression analysis showed that expression of ERCC1 mRNA and P53 protein was negative factors for curative effect of NACT（ P 〈 0. 05 ） , whereas no correlation was found between over - expression of ERCC1 mRNA,P53 and age group ,pathological grade as well as tumor type（P 〉 0.05）. Over-expression of ERCC1 mRNA was found to be positively correlated with P53 protein （P 〈 0. 05 ）. Furthermore, we also found high risk group of patients accounted for 68.9% （ 35 ） , undergoing radiotherapy in the postoperative pathological results. Conclusion The over - expression of ERCC1 mRNA and P53 protein in LACC tissue is related to the resistance to NACT,which could be used to indicate whether a high risk exists based on the post- operative pathological results. In addition, expression of two markers demonstrates positive correlation.