TIMP-1和MMP-9与胆囊癌发生、发展的关系

Expression of TIMP-1 and MMP-9 in Gallbladder carcinoma and precancerous lesions

目的探讨组织金属蛋白酶抑制剂1(TIMP-1)与基质金属蛋白酶9(MMP-9)在胆囊癌及癌前病变组织中的表达,阐明其与胆囊癌发生、发展的关系。方法应用免疫组化SP法检测TIMP-1和MMP-9在42例胆囊癌、18例胆囊上皮中重度不典型增生、13例胆囊上皮轻度不典型增生及30例慢性胆囊炎组织中的表达。结果胆囊癌组中MMP-9的阳性表达率(88.10%)较轻度不典型增生组(30.77%)及慢性胆囊炎组(20.00%)高,差异有统计学意义(P<0.0083),其表达强度差异亦有统计学意义(P<0.0083)。胆囊癌组TIMP-1的阳性表达率(90.48%)高于慢性胆囊炎组(43.33%),差异有统计学意义(P<0.0083),其表达强度差异亦有统计学意义(P<0.0083)。不同胆囊病变组织中MMP-9和TIMP-1的表达呈正相关(r=0.222,P=0.024)。慢性胆囊炎,不典型增生和胆囊癌组中的MMP-9/TIMP-l的值存在差异,差异有统计学意义(χ2=6.851,P=0.033)。结论 MMP-9和TIMP-1的阳性表达率及强度与胆囊癌的发生、发展有关,二者的比值可用来评估胆囊癌的预后。

Objective To explore the expressions of tissue inhibitors of metalloproteinase- 1（ TIMP- 1） and extracellular matrix metalloproteinase- 9（ MMP- 9） In gallbladder carcinoma and precancerous lesions and the correlations with its occurrence,progress. Methods SP immunohistochemistry was used to detect the expression of TIMP- 1 and MMP-9 in 42 cases of gallbladder carcinomas,18 cases of gallbladder epithelial atypical dysplasiaⅡ- Ⅲ tissues,13 cases of gallbladder epithelial atypical dysplasiaⅠ tissues and 30 cases of chronic cholecystitis tissues. Results The positive rates of MMP- 9 in gallbladder carcinoma group（ 88. 10%） was higher than gallbladder epithelial atypical dysplasia I group（ 88. 10%） and chronic cholecystitis group（ 20. 00%） and the difference was significant（ P 〈 0. 0083）. It is the same with the MMP- 9 expression intensity（ P 〈 0. 0083）. The positive rates of TIMP- 1 in gallbladder carcinoma group（ 90. 48%） was higher than chronic cholecystitis group（ 43. 33%） and the difference was significant（ P 〈 0. 0083）. It is also the same with the TIMP- 1 expression intensity（ P 〈 0. 0083）. The expression of MMP- 9 and TIMP- 1 was positive correlated in all the 4 groups（ r = 0. 222,P = 0. 024）. The ratio of MMP- 9 / TIMP- l were different in all the 4groups and the difference was significant（ χ~2= 6. 851,P = 0. 033）. Conclusion The positive expression rates and intensities of MMP- 9 and TIMP- 1 are correlated with gallbladder carcinoma and its occurrence,progress. The ratio of MMP- 9 / TIMP- l could be uesd to evaluate the prognosis of gallbladder carcinoma.