香樟CcCBFs基因的克隆及表达模式

Cloning and Expression Profiling of Cc CBF Genes in Cinnamomum camphora

CBF转录因子又称为DREB1,在增强植物抵御非生物胁迫(低温、干旱和盐胁迫)方面具有重要的作用。利用同源克隆的方法结合RACE PCR技术,从香樟中获得2个CBF类似基因的c DNA全长序列,命名为Cc CBFa和Cc CBFb,其c DNA序列全长分别为909和941 bp,分别包含一个714和729 bp的开放阅读框,编码237和242个氨基酸;g NDA序列分析结果显示,Cc CBFa和Cc CBFb基因均不含有内含子。一系列生物信息学分析表明,Cc CBFa和Cc CBFb基因属于DREB家族的CBF/DREB1亚家族,将所获得香樟CBF基因c DNA全长序列提交到NCBI(登录号:KJ958932和KJ958933)。此外,实时定量PCR结果表明,Cc CBFa和Cc CBFb基因在香樟不同器官中的表达量存在一定差异,并且均能被低温、干旱、盐以及ABA强烈诱导。这些结果表明,Cc CBFa和Cc CBFb可能在香樟应对低温、干旱和盐等非生物胁迫时发挥重要作用,并且可能与ABA信号通路有关。

As an important transcription factor,C-repeat binding factor（CBF）,also called dehydration-responsive element binding factor 1（DREB1）,plays a key role in enhancing plant tolerance to abotic environmental stresses such as low temperature,drought and salinity.Two novel CBF-like genes designated as CcC BFa and CcC BFb were successfully isolated from Cinnamomum camphora,and the full length cD NA sequences were 909 and 941 bp,respectively.Their open reading frames（ORF） were 714 and 729 bp,respectively,encoding 237 and 242 amino acids.There was no intron in the coding regions of the two genes.By an array of bioinformatics analysis,two genes were classified into the CBF / DREB1 subfamily,and their full-length cD NA sequences were submitted to the NCBI with accession numbers of KJ958932 and KJ958933.By qP CR,CcC BFa and CcC BFb could be dramatically induced by low temperature,drought and salinity,as well as ABA,and the expression level of the two genes had specific expression differences in different organs of C.camphora.Therefore,CcC BFa and CcC BFb were involved in response to cold,drought,salinity,and ABA-responsive signaling pathway.