多倍体萱草的离体快繁技术

Rapid in Vitro Propagation of Polyploid Hemerocallis fulva

以多倍体萱草的嫩叶、生长点、腋芽、花蕾、花瓣和花茎作外植体进行离体快繁技术研究。结果表明：以幼嫩的花蕾和花瓣为外植体最为适宜;以MS＋6-BA 0.5 mg/L＋NAA 0.2 mg/L＋2,4-D 0.7 mg/L为最适诱导培养基,诱导分化率可达74%以上;以MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L为最适继代增殖培养基,增殖系数可达5.8;以MS＋6-BA 1.0 mg/L＋NAA 0.2 mg/L＋IBA 0.02 mg/L＋马铃薯20 g/L为壮苗培养基。培养1~2周后转入MS＋NAA0.04 mg/L的生根培养基中,其平均根长、根长势等最为理想,生根率达100%,且移栽后成活率高,生长健壮。

The experiment was conducted to study the rapid in vitro propagation of polyploidy Hemerocallis fulva with tender leaf,growing point,axillary bud,bud,petal and scape as explants.The young bud and petal were the most suitable explants.MS＋6-BA 0.5 mg / L＋NAA 0.2 mg / L＋2,4-D 0.7 mg / L was the most suitable inducing medium,and the inducing differentiation rate was 74%.MS＋6-BA 2.0 mg / L＋NAA 0.2 mg / L was the most suitable subculture medium,and proliferation coefficient was 5.8.MS＋6-BA 1.0 mg / L＋NAA 0.2 mg / L＋IBA 0.02 mg / L＋Potato 20 g / L was the strong seedling medium.After 1-2 weeks,tissue culture seedling was transferred to the rooting medium of MS＋NAA 0.04 mg / L.The average root length and root growth of tissue culture seedlings were the most ideal with the rooting rate of 100%,the survival rate was high,and the growth was strong.