hrHPV感染对宫颈癌组织中抗凋亡基因、促凋亡基因表达的影响

Effect of hrHPV infection on anti-apoptotic gene and pro-apoptotic gene expression in cervical cancer tissue

目的：研究hrHPV感染情况对宫颈癌组织中抗凋亡基因、促凋亡基因表达的影响。方法：选择2013年5月~2015年12月期间在广东省佛山市南海经济开发区人民医院就诊的宫颈癌患者56例、宫颈上皮内瘤变患者94例、慢性宫颈炎患者48例进行研究,分别纳入恶性组、癌前病变组、良性组。测定宫颈组织中hrHPV感染情况以及抗凋亡基因、促凋亡基因的表达量。结果：恶性组患者宫颈组织中hrHPV的感染率以及病毒负荷量显著高于癌前病变组和良性组;恶性组宫颈组织中p27和p16的含量显著低于癌前病变组和良性组,K-ras、c-myc、Prdx4、TNFAIP8的含量显著高于癌前病变组和良性组;HPV病毒负荷量越大,恶性组宫颈组织中p27和p16的含量越低,K-ras、c-myc、Prdx4、TNFAIP8的含量越高。结论：hrHPV感染能够造成抑癌基因p27和p16表达缺失、增加原癌基因和凋亡抑制基因的表达,与宫颈癌的发生和发展有关。

[ABSTRACT]Objective：To study the effect of hrHPV infection on anti-apoptotic gene and pro-apoptotic gene expression in cervical cancer tissue.Methods：A total of 56 patients with cervical cancer,94 cases of patients with cervical intraepithelial neoplasia and 48 cases of patients with chronic cervicitis who were treated in our hospital from May 2013 to December 201 5 were selected for study and included in malignant group,precancerous lesion group and benign group respectively.hrHPV in-fection as well as the expression of anti-apoptotic genes and pro-apoptotic genes in cervical tissue were detected.Results：hrH-PV infection rate and viral load in cervical tissue of malignant group were significantly higher than those of precancerous lesion group and benign group;P27 and p1 6 levels in cervical tissue of malignant group were significantly lower than those of precan-cerous lesion group and benign group,and K-ras,c-myc,Prdx4 and TNFAIP8 levels were significantly higher than those of precancerous lesion group and benign group;the greater the HPV virus load,the lower the p27 and p1 6 levels and the highernbsp;the K-ras,c-myc,Prdx4 and TNFAIP8 levels in cervical tissue.Conclusions：hrHPV infection can result in tumor suppressor genes p27 and p1 6 expression deletion and increase the expression of proto-oncogene and apoptosis-inhibiting genes,and it is associated with the occurrence and development of cervical cancer.