化痰散结法对感染血吸虫小鼠肝脏虫卵肉芽肿炎症和纤维化的干预

The intervention of Huatan Sanjie herbal medicine on the liver of mice infected with Schistosome Eggs Granulomatous Inflammation and fibrosis

目的:探讨化痰散结中药对感染血吸虫小鼠肝组织炎症和纤维化的影响。方法:将感染18头血吸虫尾蚴致血吸虫病肝损伤的72只小鼠分为4组,模型组24只,化痰散结中药提取物高、中、低剂量组各16只;另16只正常BALA/c小鼠为正常组。制备50mg/kg、100mg/kg、150mg/kg、中、高3种剂量化痰散结中药提取物(浙贝母、漏芦由乙醇提取),第6周开始对高、中、低剂量组小鼠灌胃不同剂量化痰散结中药提取物0.5ml/次,1次/d,疗程8周;正常组和模型组小鼠灌胃等量蒸馏水,剂量疗程同上。实验结束时取眼球血检测血清ALT、AST,然后颈椎脱臼处死小鼠留取肝组织标本,分别观察肝脏病理和检测18kDa转运蛋白(TSP0)分布与表达。结果:感染第6周时模型组小鼠肝组织虫卵肉芽肿结节形成,汇管区纤维组织增生,炎症反应明显,说明模型建造成功。实验结束时,低、中、高剂量组小鼠血清ALT、AST和肝组织虫卵肉芽肿平均面积均明显低于模型组(P<0.05),其中各指标均以高剂量组最好,中剂量组次之(P<0.05);免疫组化染色显示模型组小鼠肝组织TSPO表达多于正常组,高、中、低剂量组小鼠肝组织TSPO表达明显低于模型组。结论:浙贝母、漏芦乙醇提取物能减轻血吸虫小鼠肝组织炎症和纤维化程度,其机制可能与抑制炎性细胞和肝星状细胞TSPO的表达有关。

Objective：To explore the effect of Huatan Sanjie herbal medicine on the liver of mice infected with Schistosome Eggs Granulomatous Inflammation and fibrosis.Methods：The models of liver injury induced by schistosoma were established by exposure of mice percutaneously through the tail to cercariae of schistosoma and Huatan Sanjie decoction（the ethanol extract of fritillaria and rhaponticum uniflorum）were produced into three kinds of low,medium and high doses（50mg/kg,100mg/kg,150mg/kg）.The mice were randomly divided into five groups.From the beginning of the sixth week,we began to treat the mice with low,middle and high dose Chinese herbs of Huatan Sanjie.The period of Treatment were eight weeks.After the end of treatment,we collected serum samples for one to two milliliter and killed the mice by Cervical dislocation method.Then we got the samples of livertissue by the dissection of mice.The serum transaminase were detected by automatic biochemical analyzer and histological sections of liver were evaluated by hematoxylin and eosin stains.The TSPO were examined by method of Immune histochemical.Results：In the sixth week,we observed the histological sections of liver which were stained by hematoxylin and eosin and found the formation of egg granulomas and the regeneration of nodules,we also found fibrous connective tissue hyperplasia and inflammatory response in the portal of the liver.These showed that the models of liver fibrosis induced by schistosoma were established successfully.After the end of the treatment,compared with the model group,the level of serum ALT,AST and the areas of egg granuloma in treatment groups decreased obviously（P〈0.05）.Among them,all the indexes were the best in the high dose group,followed by middle dose group（P〈0.05）;After the end of the treatment,compared with the normal control group,the expression of TSPO in the model control group were significantly increased and then decreased obviously by treating with different kind doses of Chinese herbs of Huatan Sanjie.Conclusion：The ethanol extract of fritillaria and rhaponticum uniflorum had obviously inhibitory effect on the inflammation and fibrosis of liver tissue induced by schistosoma infection.The mechanisms maybe related to restraining the expression of TSPO in Inflammatory cell and hepatic stellate cell.