骨髓间充质干细胞移植对Tourette综合征大鼠纹状体脑源性神经营养因子表达的影响

Influence of mesenchymal stem cell transplantation on brain-derived neurotrophic factor expression in rats with Tourette syndrome

目的考察骨髓间充质干细胞(MSC)移植对Tourette综合征(TS)大鼠纹状体内脑源性神经营养因子(BDNF)表达的影响,探讨MSC移植治疗TS的作用机制。方法采用立体定向技术向大鼠纹状体内微量灌注抗基底神经节抗体(ABGA),制备自身免疫TS动物模型;采用密度梯度离心法在体外分离、培养和扩增大鼠MSC,经流式细胞仪鉴定后将MSC标记并移植到TS大鼠纹状体内。通过ELISA及RT-PCR技术检测大鼠脑内BDNF水平。结果将MSC移植到TS大鼠纹状体内,自移植后7d开始观察到TS大鼠的刻板行为减少。TS大鼠纹状体内BDNF水平升高(P<0.05);MSC移植后TS大鼠纹状体内BDNF含量下降。结论 MSC移植能减少TS大鼠的刻板行为、降低TS大鼠体内的BDNF含量。

Objective To investigate bone marrow mesenchymal stem cells（MSC）transplantation on brain-derived neurotrophic factor（BDNF）expression in the striatum of Tourette syndrome（TS）rat,and to explore the possible mechanism of MSC transplantation in the treatment of TS. Methods Seventy-two Wistar rats were randomly allocated to the control group and the two experimental groups,namely,TS rats＋vehicle and TS rats＋MSC.MSCs were co-cultured with 5-bromodeoxyuridine（BrdU）for 24 hfor labeling prior to grafting.An autoimmune TS animal model was adopted in the present study.Rat MSCs were cultured and expanded using density gradient centrifugation in vitro,identified by flow cytometry and transplanted into the striatums of TS animal.BDNF level was detected by ELISA and RT-PCR techniques. ResultsStereotypic behaviors reduced in TS rats 7days after MSC transplantation into rat striatum.BDNF level in striatum of TS rat increased（P〈0.05）.After MSC transplantation,the BDNF level decreased in the striatum of TS rat. Conclusions Intrastriatal transplantation of MSCs can provide relief from the stereotypic behavior of TS.BDNF level reduces in TS rats after MSC transplantation.