淫羊藿和女贞子提取物对骨质疏松症大鼠性激素功能水平的影响

Effect on sex hormone functional of extracts of Herba Epimedii/Fructus Ligustri Lucidi in osteoporosis rats

目的:探讨淫羊藿和女贞子提取物对骨质疏松症(OP)大鼠性激素功能水平的影响。方法:50只3月龄雄性Wistar大鼠随机分为正常组、模型组、提取物低剂量组、提取物中剂量组、提取物高剂量组共5组,每组10只,除正常组外,其他4组以维甲酸灌喂15 d制作OP模型,造模成功后正常组和模型组灌服生理盐水,提取物低剂量组、中剂量组和高剂量组分别按50 mg/kg、100 mg/kg、200 mg/kg灌服淫羊藿和女贞子提取物混合物,均持续3周。测量各组骨密度(BMD),左侧股骨切片采用苏木精和伊红(HE)染色观察股骨病理学改变并测定骨小梁组织形态相关参数,免疫组织化学分析激素受体蛋白表达。结果:正常组股骨全部、股骨上端、股骨下端BMD显著高于模型组,提取物中剂量组和高剂量组股骨全部、股骨上端、股骨下端BMD显著高于模型组,提取物低剂量组股骨全部BMD显著高于模型组,差异均有统计学意义(P<0.05或P<0.01);与模型组比较,提取物各组骨小梁数量有了显著增加,完整性、连续性明显好转,相关参数比较结果显示,正常组Tb.N、Tb.Ar、Tb.Th显著大于模型组,Tb.Sp显著小于模型组,提取物中剂量组和高剂量组Tb.N、Tb.Ar、Tb.Th显著大于模型组,Tb.Sp显著小于模型组,提取物低剂量组,Tb、Ar、Tb、Th显著大于模型组,差异均具有统计学意义(P<0.05或P<0.01);正常组AR、ER蛋白表达量明显高于模型组,而提取物高剂量组AR、ER蛋白表达量较模型组显著上升,差异有统计学意义(P<0.05或P<0.01)。结论:淫羊藿和女贞子提取物能够增加维甲酸诱导的OP大鼠BMD,逆转骨组织病理结构,上调性激素受体蛋白表达,对维甲酸诱导的OP大鼠具有潜在的保护作用。

Objective： To explore the effect on sex hormone functional of extracts of Herba Epimedii/Fructus Ligustri Lucidi in osteoporosis rats. Methods： 50 cases of 3 months Wistar rats were rando mly divided into normal group,model group,extract low dose group（ extract-L）,extract middle dose group（ extract-M）,extract high dose group（ extract-H）,10 cases in each group,in addition to the normal group,other 4 groups were bulided OP model by filling and feeding retinoic acid for 15 d,then normal group and model group were filling physiological saline; extract-L,extract-M,extract-H were respectively feeding extracts of Herba Epimedii / Fructus Ligustri Lucidi at 50 mg / kg,100 mg / kg,200 mg / kg,both for 3 weeks. Measured the bone mineral density（ BMD）,observed the femoral pathology change of left femur with hematoxylin and eosin（ HE） staining,measured trabecular bone tissue morphology related parameters,analyzed the hormone receptor protein expression with immunohistochemistry. Results： The BMD of the whole femur,femur head,and femur neck in normal group were all significantly higher than that of model group,the BMD of the whole femur,femur head,and femur neck in extract-M and extract-H group were all significantly higher than that of model group,the BMD of the whole femur in extract-L group was significantly higher than that of model group,the differences were statistically significant（ P〈0. 05 or P〈0. 01）. The bone trabecular number had significantly increased,integrity,continuity improved significantly,bone histomorphometry such as Tb. Ar,Tb. Th,and Tb. N in model group were decreased,and Tb. Sp was increased,extract-M and extract-H group had remarkable effect on each index,while extract-L group only had a remarkable effect on Tb. Ar and Tb. Th（ P〈0. 05 or P〈0. 01）,compared with model group. The expression of AR,ER in normal groups were significantly higher than that of model group,the expression of AR,ER in extract-H group was significantly higher than that of model group,the differences were statistically significant（ P〈0. 05 or P〈0. 01）.Conclusion： This study demonstrated that the combination with TFE and TIFL had potential protective effects on osteoporosis rats induced by retinoic acid. The combination with TFE and TIFL is able to increase BMD,reverse pathological changes of bone tissue,coordinate hormones of the hypothalamic-pituitary-gonadal axis,and upregulate the expression of sex hormone receptors.