姜黄挥发油对黑色素瘤wm9细胞增殖与凋亡的影响

Effect of turmeric volatile oil on proliferation and apoptosis of melanoma wm9 cells

目的研究姜黄挥发油对黑色素瘤wm9细胞增殖与凋亡的影响。方法应用不同浓度的姜黄挥发油作用于黑色素瘤的wm9细胞。用细胞增殖计数试剂盒(CCK8)检测增殖抑制率,倒置显微镜观察细胞形态学变化,ELISA法检测Caspase-3酶活性,流式细胞检测细胞周期和凋亡。结果姜黄挥发油在不同浓度下,对黑色素瘤wm9细胞作用表现不同,当药物浓度达到40mg/L,倒置显微镜下可见到典型的凋亡细胞;Caspase-3酶活性随着药物浓度的递增,呈现药物浓度-酶活性正相关性;经姜黄挥发油作用后的wm9细胞被阻滞在G_2/M期。结论姜黄挥发油诱导黑色素瘤wm9细胞凋亡的机制与细胞周期G2/M期阻滞、Caspase酶活化参与凋亡有关。

Objective To investigate the effect of turmeric volatile oil on the proliferation and apoptosis of melanoma wm9 cells. Methods Different concentration of turmeric volatile oil were applied on melanoma win9 cells;Detection of proliferation inhi- bition rate by cell counting kit-8（CCK8） ;Morphological changes of cells were observed by inverted microscope;Caspase-3 activities were evaluated by enzyme linked immunosorbent assay（ELISA） ;Celt cycle and apoptosis was analyzed with flow cytometry（FCM）. Results The effects of turmeric volatile on melanoma wm9 cells were different in different concentrations,when the drug concen- tration reached at 40 mg/L, typical apoptotic cells can be seen under the inverted microscope,Caspase-3 activities were positively correlated with drug concentration. Cell cycle of Win9 cells were blocked in G2/M phase after exposed to turmeric volatile o＇il. Con- clusion Turmeric volatile oil has capability of apoptois-inducing in Wm9 cells, Moreover,turmeric volatile oil activated the key en- zyme of apoptosis pathway and arrested cell cycle in G2/M phase,which maybe the mechanism of turmeric volatile oil to inhibit pro- liferation and differentiation of tumor cells.