他汀干预对兔主动脉瓣退行性病变影响的研究

Effect of statins on the degenerative changes of aortic valve in rabbits

目的:建立实验性高脂血症动物模型,观察高脂血症能否直接诱导兔主动脉瓣膜发生退行性主动脉瓣疾病(DAVD)的病理改变,以及他汀干预对瓣膜病变的影响。方法:选择健康新西兰纯种雄性白兔39只,随机平均分配为正常对照组(N组),高脂饮食组(HC组),高脂饮食加阿托伐他汀干预组(AI组)。分别于实验开始,4,8,12周后,取静脉血测定血浆脂质水平,分析比较各组间是否存在差异,同时取主动脉瓣膜标本行组织学检测。结果:HC组和AI组血浆中胆固醇(CHOL)、TG、LDL-C、C反应蛋白(CRP)的浓度第8,12周,较4周及N组均升高,同期AI组的各项血脂水平低于HC组,差异有统计学意义(P<0.05);HDL-C、CK、ALT实验前后及三组间,差异无统计学意义(P>0.05)。HC组主动脉瓣膜的光镜HE染色及电镜病理表现与DAVD早期病理组织学改变相似,AI组病变程度较HC组减轻。结论:高脂饮食能够诱导兔主动脉瓣膜发生与早期DAVD相似的病理组织学改变。阿托伐他汀能够明显延缓DAVD病变发展。

Objective： To observe whether the hyperlipemia can directly induce aortic valve degenerative aortic valve disease（ DAVD） pathological changes of experimental rabbits and effects of atorvastatin on mitral valve disease. Methods： 39 New Zealand white rabbits were randomly divided into normal control group（ N group）,high fat diet group（ HC group） and high fat diet plus atorvastatin group（ group AI）. The level of plasma lipid of rabbits were respectively tested at beginning of 4^（th）,8^（th） and 12^（th） weeks. The date were compared between groups to obserbe whether there is significant statistical difference. Meanwhile the specimens of aortic valve were operated for histological examination. Results： The plasma glycerol cholesterol（ Chol）,TG,LDL,C reactive protein（ CRP） concentration of HC and AI group at the 8^（th） and 12 ^（th） weeks were ubcrease compared to N group and the end of 4thweek. At the same period the serum lipid level of AI group was lower than the HC group. Statistical analysis showed significant difference（ P 0. 05）; HDL,CK,ALT between 3 groups,pre and post the experiment showed no significant difference（ P 0. 05）. Compared with the HC group,the degree of aortic valve lesions of group AI was reduces. Conclusion：（ 1） High fat diet can induce the rabbit aortic valve to pathological changes which similar to the early DAVD pathology.（ 2） Atorvastatin can delay the development of DAVD lesions.