pH示差法测定不同品系元宝枫叶片花青苷含量

Total Anthocyanins Content in Different Strains of Acer truncatum Bunge by pH-Differential Method

采集秋季转色后的元宝枫无性系叶片作为试验材料,采用pH示差法测定其不同品系叶片中花青苷的含量。结果显示:用恒温震荡培养箱25℃震荡提取6 h提取效果最好;pH示差法选定pH值是1.0和4.5,提取液的平衡时间是60 min。试验的平均回收率是96.66%,由此可见,该方法能有效消除溶液中杂质对测定结果的影响,可用于元宝枫花青苷的定量分析。在所测量的5个品系中,花青苷含量最高的品系是1-7号,含量为0.845 3 mg·g-1,5个品系的平均花青苷含量是0.482 1 mg·g-1。

In this study, the leaves of Acer truncatum Bunge were studied, and their total anthocyanin contents in different strains were investigated by pH-differential method. The results showed that using constant temperature oscillation incubator 25 ℃ oscillation extraction 6 hours it presented the best effect, and the pH selected were 1.0 and 4.0, and the buffering time was 60 minutes. By this method, the average recovery rate was 96.66%,therefore, this method can be used to the quantitative analysis of anthocyanins in Acer,and pH-differential method could eliminated the influence of impurities. Among the five strains,the content of anthocyanin in 1-7was the highest,which was 0.845 3 mg·g-1; the average anthocyanin content of these five strains was 0.482 1 mg·g-1.