枸杞子多糖通过改善氧化应激延缓疲劳作用的研究

Anti- fatigue activity of Lycium barbarum polysaccharides in mice by attenuating oxidative stress

本文研究枸杞子多糖(LBP)对小鼠疲劳的延缓作用及机制。将昆明种小鼠分为空白对照组(给予生理盐水)和高、中、低剂量枸杞子多糖处理组(剂量分别为100、50和25 mg/kg/day),灌胃给药2周,通过跑步、疲劳转棒和负重游泳实验考察枸杞子多糖对小鼠的抗疲劳作用,并对运动后小鼠体内肝糖原、肌糖原以及血清、肝脏和肌肉中抗氧化指标进行检测。实验结果表明,LBP可以明显延长小鼠的运动时间,增加小鼠肝糖原和肌糖原含量,同时明显提高血清、肝脏和肌肉中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)的活性,显著降低肝脏和肌肉中丙二醛(MDA)和活性氧(ROS)含量及血清中MDA含量。LBP具有延缓疲劳的作用,其机制可能是减弱氧化应激和提高糖原含量。

The effect of Lycium barbarum polysaccharide（LBP） on delaying fatigue via attenuating oxidative stress was studied in mice model.After 2-week LBP treatment with dose of 25,50 and 100 mg/kg,exercise time of the mice was detected via rotating rod test,forced swimming test,and forced running test.After 20 min swimming,the levels of hepatic glycogen, muscle glycogen, and some antioxidant indexes were detected in serum, liver, and muscle.LBP obviously prolonged exercise time, increased the levels of liver glycogen and muscle glycogen, enhanced the activities of SOD and GSH- Px, and decreased the levels of MDA and ROS in serum, liver and muscle.The anti-fatigue activity of LBP may be associated with attenuating oxidative, further increasing glycogen content in the mice.