中药杜仲-牛膝配伍对去卵巢大鼠骨质疏松症的干预实验研究

Influence of TCM Eucommia-Achyranthes Compatibility on Osteoporosis in Ovariectomized Rats

目的探讨杜仲-牛膝配伍组对去卵巢骨质疏松大鼠模型的干预作用。方法将84只大鼠随机分为正常对照组12只,假手术组12只,剩余60只造模成功后随机分为:模型组、阳性对照组(阿法骨化醇组)、杜仲组、牛膝组、杜仲-牛膝组共5组各12只。给药3个月后,检测各组大鼠的血清E2、钙离子、磷离子、碱性磷酸酶和骨密度(the bone mineral density,BMD)。结果与正常对照组相比,模型组大鼠血清中雌二醇(estradiol,E2)、钙离子、磷离子及BMD值明显降低(P<0.01),血清中碱性磷酸酶含量明显增高(P<0.05)。与模型组相比,牛膝组、杜仲-牛膝组大鼠血清中E2的含量明显升高(P<0.05);杜仲组、牛膝组、杜仲-牛膝组、阳性对照组大鼠BMD值及血清中钙离子、磷离子均明显升高(P<0.01)。杜仲组、牛膝组、杜仲-牛膝组、阳性对照组大鼠血清中碱性磷酸酶含量均明显降低(P<0.05);杜仲-牛膝组血碱性磷酸酶明显低于杜仲组、牛膝组(P<0.05)。结论杜仲-牛膝药对抗骨质疏松作用有增强趋势,其作用可能与提高血清钙离子、磷离子浓度,提高BMD有关。

Objective To investigate the interventional effect of Eucommia-Achyranthes compatibility on osteoporosis in ovariectomized rats. Methods Eighty-four rats were randomly divided into normal control group （n=12）, sham-operation group （n=12）, and the remaining 60 rats after the success of the modeling were randomly divided into model group, positive control group （group afar ossification alcohol）, eucommia bark group, achyranthes group, eucommia-achyranthes group, 12 rats in each group. After given medicine for 3 months, the serum estradiol （E2）, calcium ion, phosphate ion, alkaline phosphatase and the bone mineral density （BMD） were detected. Results Compared with normal control group, the serum E2, calcium ion, phosphate ion and bone mineral density of model group were significantly lower than normal control group （P〈0.01）, and the alkaline phosphatase in serum was more higher （P 〈0.05）. Compared with the model group, the content of E2 in achyranthes group and eucommia-achyranthes group was increased obviously （P〈0.05）, the BMD and serum calcium ion, phosphate ion in eucommia bark group, achyranthes group, eucommia-achyranthes group, postive control group were increased obviously （P〈0.01）, and the alkaline phosphatase in the groups were reduced significantly （P〈0.05）. The alkaline phosphatase of eucommia-achyranthes group was lower than that in eucommia bark group and aehyranthes group （P〈0.05）. Conclusion The comparability of Eucommia-Aehyranthes shows increasing trend in anti-osteoporosis, and its effect may be related to increasing serum calcium, phosphorus, improving BMD.