基于可编程LCD的莱茵伯格照明显微原理与系统设计

Theory and Systematic Design of Rheinberg Illumination Microscopy Based on Programmable LCD

莱茵伯格照明是显微成像中光学染色的一种方式,照明光源通过特殊的滤色片使样品与背景产生色差,有效提高无色透明样品的对比度。基于可编程液晶显示器(LCD)的莱茵伯格照明显微系统将传统显微镜中聚光镜光阑替换成低成本的薄膜晶体管液晶显示器只需改变其照明图案,就能够实现明场、暗场、相位差、倾斜成像以及莱茵伯格照明等多种显微成像功能。并且由于LCD面板的颜色与光强灵活可调,系统可以衍生出彩虹暗场、彩虹相差等全新的光学染色方法。采用该系统对未染色的肺癌细胞、纺织纤维、小鼠肾脏切片等无色透明样品进行显微观察,验证了系统的多样性与可靠性。

Rheinberg illumination is an optical staining technique for microscopic imaging.The light passing through special filters provides chromatism between the specimen and background,which improves the contrast of colorless and transparent samples effectively.Rheinberg illumination microscopy based on programmable liquid crystal display（LCD）replaces the condenser diaphragm of conventional microscope with low-cost thin film transistor liquid crystal display.By displaying different patterns,numerous microscopy imaging modalities,such as bright field,dark field,phase contrast,oblique illumination and Rheinberg illuminations can be realized.Furthermore,because it is easy to modulate both the color and the intensity of the LCD panel,the system derives completely new methods for optical staining,such as iridescent dark-field and iridescent phase-contrast imaging.The versatility and effectiveness of the system are demonstrated by microexamination of several transparent colorless specimens,such as unstained lung cancer cells,textile fibers,and slice of mouse kidney.