舌下特异性免疫治疗对尘螨所致变应性鼻炎患者IL-17和IL-35的表达水平及Treg/Th17细胞平衡的影响

Effects of specific immunotherapy on the expression levels of serum IL-17,IL-35 and Treg/Th17 regulatory T cellsin patients with allergic rhinitis caused by dermatophagoides

目的:研究尘螨阳性变应性鼻炎(AR)患者舌下特异性免疫治疗(SLIT)前后外周血中IL-17、IL-35的表达水平及对Treg/Th17细胞平衡的影响。方法:选取30例确诊为尘螨阳性并接受SLIT的AR患者为AR组,30例健康体检者为对照组。采用ELISA检测对照组及AR组治疗前后血清中的IL-35和IL-17的表达水平;采用流式细胞术检测对照组及AR组治疗前后外周血中CD4^+CD25^+Foxp3^+T细胞及CD4^+IL-17^+Th17细胞占CD4^+T细胞的百分率。结果:AR组治疗前IL-17的表达水平明显高于对照组(P<0.05),治疗后较治疗前明显下降(t=5.030,P<0.05),但仍高于对照组(P<0.05)。AR组治疗前IL-35的表达水平明显低于对照组(P<0.05),治疗后较治疗前上升(t=-4.083,P<0.05),但仍低于对照组(P<0.05)。AR组治疗前CD4^+CD25^+Foxp3^+Treg细胞占CD4^+T细胞的百分率明显低于对照组(P<0.05),治疗后较治疗前上升(t=-10.584,P<0.05),但仍低于对照组(P<0.05)。AR组治疗前CD4+IL-17+Th17细胞占CD4^+T细胞的百分率明显高于对照组(P<0.05),治疗后较治疗前下降(t=6.258,P<0.05),但仍高于对照组(P<0.05)。结论:SLIT能够对AR患者外周血中IL-17、IL-35的表达水平及Treg/Th17细胞的平衡产生影响。

Objective：To explore the regulatory effect of sublingual immunotherapy on the balance of Treg/ Th17 cells and the expression of IL-17 and IL-35 in serum of allergic rhinitis（AR） in pre-specific and post-specific immunotherapy. Method：In this study, 30 cases were randomly selected from outpatients of otolaryngological department in the second hospital of Hebei Medical university. These were attributed as pretherapy group. After treatment , the same patients were as renamed as the post therapy group. Another 30 cases were healthy subjects enrolled from physical examination branch of our hospital. We detected the expression level of IL-35 and IL-17 in peripheral blood by using ELISA and defeced CD4^＋ CD25^＋ Foxp3^＋ T cell and CD4^＋ IL-17^＋ T cell expression level via flow cytometry. Result： The expression level of IL-17 in pre-therapy group was obviously higher than that in control group（P〈0.05 ） ; The expression level of IL-17 in post-therapy group was obviously lower than that in pre-therapy group, The difference was a statistically significance （t= 5. 030, P〈0.05） ;The expression level of IL- 17 in post-therapy group was also higher than that in control group（P〈0.05 ）. The expression level of IL-35 in pre-therapy group was obviously lower than that in control group（P〈0.05 ）;The expression level of IL-35 in post-therapy was obviously higher than that in pre-therapy group; The difference was a statistically significance （t=-4. 083,P〈0.05）,the expression level of IL-35 in post-therapy group was also lower than that in control group（P〈0.05）. The percentage of CD4^＋ CD25^＋ Foxp3^＋ T cell in CD4^＋ T cell was significant lower in pre-therapy group than that in control group （P〈0.05） ; The percentage of CD4^＋ CD25^＋ Foxp3^＋ T cell in CD4^＋ T cell in post-therapy was obviously higher than that in pre-therapy group; The difference was a statistically significance （t=-10. 584,P〈0.05）,The percentage of CD4^＋ CD25^＋ Foxp3^＋ T cell in CD4^＋ T cell was also lower in post- therapy group than that in control group （P〈0.05）. The percentage of CD4^＋ IL-17^＋T cell in CD4^＋ T cell was significant higher in pre-therapy group than that in control group （P〈0.05） ; The percentage of CD4^＋ IL-17^＋ T cell in CD4^＋ T cell in post-therapy group was obviously lower than that in pre-therapy group. The difference was a statistically significance （t=6. 258,P〈0.05）. The percentage of CD4^＋ IL-17^＋ T cell in CD4^＋ T cell was also higher in post-therapy group than that in control group （P〈0.05）. Conclusion： Specific immunotherapy can have an impact on the expression levels of IL-17, IL-35 and also on Treg/Th17 cells balance in peripheral blood for patients with allergic rhinitis.