精清浓度对马精子低温及冷冻保存效果的影响

Effect of Seminal Plasma Concentration on the Quality of Cooled Stored and Frozen-thawed Equine Semen

本研究旨在比较不同精清浓度对马精子低温保存效果的影响。选取4匹4～12岁英纯血种公马,收集精液用含有不同浓度精清的稀释液进行精液低温保存或冷冻,检测保存和解冻后精液质量,评价精清浓度对马精子低温和冷冻保存效果的影响。结果表明：30%和40%浓度精清组低温保存后精液TM、PM、RAP及VAP值均显著低于0%、10%和20%精清浓度组（P〈0.05）,10%精清浓度组低温保存24 h后TM值显著高于0%和20%精清浓度组,但保存至96 h后精液TM显著低于0%精清浓度组。不同浓度精清对精液冷冻效果的研究结果表明,含0%浓度精清组冻融后精液质量最优,随着精清浓度的增加冻融后活力及质膜完整性逐级递减。综上数据认为,进行马精液低温或冷冻保存时不添加精清可获得最理想的保存结果。

The present study was designed to compare the impact of seminal plasma concentrations on the quality of cooled stored and frozen-thawed equine semen. We select 4 4-12 years old thoroughbred stallions, collect semen and place in extender containing different concentrations of seminal plasma to store or freeze, and then evaluate the quality of semen after storage 1, 24, 48, 72 and 96 h or thawing. The results showed that TM, PM, RAP and VAP of addition of 30% and 40% seminal plasma were significantly lower than 0%, 10% and 20% group P〈0.05）, TM of addition of 10% seminal plasma was significantly higher than 0% and 20% group after cooled-stored 24 h, while the control group （0%） was significantly higher than 10% after cooled-stored 96 h. The effect of addition of seminal plasma on cryopreservation showed dose-dependence, Accompanied by increasing concentration of seminal plasma post-thaw TM, PM, RAP and viability gradually decreasing.