海洋卡盾藻对青岛大扁藻的化感作用及其对UV-B辐射的响应

ALLELOPATHIC EFFECT OF CHATTONELLA MARINA ON PLATYMONAS HELGOLANDICA VAR. TSINGTAOENSIS AND THE RESPONSE TO UV-B RADIATION

本研究以海洋微藻——赤潮藻海洋卡盾藻(Chattonella marina)与饵料藻青岛大扁藻(Platymonas helgolandica var.tsingtaoensis)为研究对象,采用单培养和共培养的方法,研究二者间的竞争关系,并在此基础上研究海洋卡盾藻去藻过滤液和藻细胞裂解液对青岛大扁藻的化感作用,同时进一步探讨两种海洋微藻间的化感作用对UV-B辐射增强的响应。结果显示:共培养条件下,海洋卡盾藻在2个高浓度下对青岛大扁藻的生长产生显著抑制(P<0.05);海洋卡盾藻去藻过滤液和藻细胞裂解液对青岛大扁藻的生长也具有显著抑制作用(P<0.05),且藻细胞裂解液的抑制作用更强,说明海洋卡盾藻对青岛大扁藻产生化感作用,且通过细胞间直接接触传递的化感物质多于通过介质传递的。不同密度比例的2种藻共培养组用UV-B辐射(2.16J/m2)处理后,海洋卡盾藻对青岛大扁藻生长的化感作用有所减弱。

We investigated the growth of Chattonella marina and Platymonas helgolandica in mono-culture and co-culture and the effects of cell-free medium and lysis liquid of C. marina on the growth of P. helgolandica, and studied the responses of the two algae to UV-B radiation. Results show that in the co-culture system, C. marina inhibited the growth of P. helgolandica at two relatively higher concentrations（P〈0.05）. Both the cell-free medium and lysis liquid of C. marina showed significant inhibitory effects on the growth of P. helgolandica（P〈0.05）, and the inhibition of lysis liquid was more significant, indicating that the allelopathic effect of C. marina on P. helgolandica was realized mainly by intracellular allelochemicals other than cell contact. In addition, UV-B radiation treatment on the two algae at 2.16 J/m^2 weakened the allelopathy of C. marina on P. helgolandica.