响应曲面法优化Gluconobacter melanlgenu X42培养基

Gluconobacter melanogenus X42 Medium Optimized by Response Curved Surface Methodology

采用Plackett-Burman设计、最陡爬坡试验和中心组合试验相结合的策略对Gluconobacter melanlgenu X42转化培养基进行优化。试验结果表明:最佳转化培养基组成为麦芽抽提物0.46 g/L、蛋白胨2.07 g/L、Ca CO30.70 g/L、Zn SO40.04 g/L。优化后L-山梨糖生成量提高了9.9%。可以应用响应曲面法对G.melanlgenu X42转化培养基进行优化。

The transformation medium of Gluconobacter melanogenus X42 was optimized with the tactics of PlackettBurman design,slope-climbing test and central combination test.The experimental results showed that the optimum transformation medium components were： malt extract 0.46 g / L,peptone 2.07 g / L,Ca CO30.70 g / L and Zn SO40.04 g / L.L-sorbose production was increased by 9.9% after the optimization.G.melanogenus X42 transformation medium could be optimized by the response curved surface methodology.