摘要
采用Plackett-Burman设计、最陡爬坡试验和中心组合试验相结合的策略对Gluconobacter melanlgenu X42转化培养基进行优化。试验结果表明:最佳转化培养基组成为麦芽抽提物0.46 g/L、蛋白胨2.07 g/L、Ca CO30.70 g/L、Zn SO40.04 g/L。优化后L-山梨糖生成量提高了9.9%。可以应用响应曲面法对G.melanlgenu X42转化培养基进行优化。
The transformation medium of Gluconobacter melanogenus X42 was optimized with the tactics of PlackettBurman design,slope-climbing test and central combination test.The experimental results showed that the optimum transformation medium components were: malt extract 0.46 g / L,peptone 2.07 g / L,Ca CO30.70 g / L and Zn SO40.04 g / L.L-sorbose production was increased by 9.9% after the optimization.G.melanogenus X42 transformation medium could be optimized by the response curved surface methodology.
出处
《微生物学杂志》
CAS
CSCD
2016年第4期67-70,共4页
Journal of Microbiology
基金
辽宁省教育厅一般项目(L2013380)
辽宁省教育厅高等学校创新团队项目(LT2014023)
关键词
生黑葡萄糖酸杆菌
培养基
响应曲面法
L-山梨糖
条件优化
Gluconobacter melanogenus
medium
response curved surface methodology
L-sorbose
conditions optimization