摘要
目的:探讨Siewert Ⅱ型食管胃交界腺癌组织中人表皮生长因子受体2( HER-2)的表达及其临床意义。方法采用免疫组织化学染色( IHC)检测180例Siewert Ⅱ型食管胃交界腺癌患者肿瘤组织中HER-2的表达,并对IHC检测结果为阳性(3+)和弱阳性(2+)的肿瘤组织进行荧光原位杂交(FISH)检测,了解其基因扩增情况。结果180例Siewert Ⅱ型食管胃交界腺癌中,HER?2表达阳性21例(11.7%),弱阳性16例(8.9%),阴性(1+/0)143例(79.4%)。 HER-2阳性和弱阳性表达肿瘤组织中FISH检测的阳性率分别95.2%(20/21)和18.8%(3/16)。 IHC与FISH检测HER?2阳性表达的一致性为95.2%。 HER?2的阳性表达与Siewert Ⅱ型食管胃交界腺癌的分化程度有关( P<0.05),而与患者年龄、性别、pT 分期、pN 分期、pM 分期及 pTNM 分期无关(均 P>0.05)。 HER-2阳性的Siewert Ⅱ型食管胃交界腺癌患者的中位总生存时间( OS)为13.0个月,明显短于HER?2弱阳性和阴性的患者(21.0个月,P<0.01)。结论 Siewert Ⅱ型食管胃交界腺癌组织中HER?2的阳性表达率为11.7%,其阳性表达与肿瘤的分化程度有关。 IHC可作为Siewert Ⅱ型食管胃交界腺癌HER?2阳性表达的初筛检查,但仍应以FISH检测作为更可靠的检测方法。
Objective To investigate the human epidermal growth factor receptor-2 ( HER-2 ) expression in esophagogastirc junction adenocarcinoma ( Siewert type Ⅱ) and its clinical significance. Methods A total of 180 patients with esophagogastric junction adenocarcinoma ( Siewert type Ⅱ) were included in this study. The HER-2 expression was detected by immunohistochemistry ( IHC ) , and fluorescence in situ hybridization ( FISH) analysis was performed to assess the HER-2 gene amplification in the IHC-positive and IHC-weak positive cases. Results HER-2 overexpression ( 3+) , weak positive ( 2+) and negative (1+/0) was 11.7%(21/180), 8.9%(16/180), and 79.4%(143/180), respectively. The FISH analysis showed HER-2 gene amplification in 95.2%(20/21) of HER-2(3+) cases and 18.8% (3/16) of HER-2( 2+) cases. The concordance rate between IHC and FISH was 95. 2%. Overexpression of HER-2 (3+) was associated with the tumor differentiation (P〈0.05), and irrelevant to age, sex, pT stage, pN stage, pM stage and pTNM stage ( P〈0.05) . The median overall survival time ( OS) was 13 months in HER-2( 3+) patients, significantly shorter than the 21 months in HER-2 ( 2+) and HER-2 (+/-) cases ( P〈0.01) . Conclusions Approximately 11. 7% of the patients with esophagogastric junction adenocarcinoma ( Siewert type Ⅱ) are HER-2-positive on IHC. HER-2 overexpression is associated with the tumor differentiation. IHC can be used as a screening test for the positive expression of HER-2 in the esophagogastirc junction adenocarcinoma ( Siewert typeⅡ) . However, FISH detection can be used as a more reliable detection method.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
2016年第9期667-671,共5页
Chinese Journal of Oncology
关键词
人表皮生长因子受体2
食管胃交界腺癌
SiewertⅡ型
临床病理特征
预后
Human epidermal growth factor receptor-2
Esophagogastric junctionadenocarcinoma
Siewert type Ⅱ
Clinicopathological features
Prognosis