RP-HPLC法测定荟酚杀菌防腐剂中芦荟苷及茶多酚的含量

Determination of Aloin and Tea Polyphenols in Hui Fen Preservatives by RP-HPLC

建立了反相高效液相色谱（RP-HPLC）测定荟酚杀菌防腐剂中有效成分芦荟苷、茶多酚的含量测定的方法。采用ZORBAX SB-C_（18）色谱柱（4.6 mm×250 mm,5μm）,流动相分别为乙腈-水、0.1%磷酸水溶液-甲醇,流速为0.70 m L/min、检测波长分别为355、279 nm,柱温35℃。结果表明,芦荟苷在0.044～0.264 mg/m L（r=0.999 2）、茶多酚在0.081 6～2.937 6 mg/m L（r=0.999 1）范围内,其质量浓度与峰面积呈良好的线性关系;芦荟苷的平均加样回收率为98.25%,相对标准偏差RSD为2.07%、茶多酚的平均加样回收率为100.31%,相对标准偏差RSD为1.42%（n=5）,3个不同批次的荟酚杀菌防腐剂中芦荟苷、茶多酚的平均含量分别为0.095、0.090 mg/m L。该方法操作简便、准确度高、重现性好,可作为荟酚杀菌防腐剂中有效成分芦荟苷及茶多酚的含量测定方法。

A reversed-phase high performance liquid chromatography（RP-HPLC） method for determination of the content of effective components of aloin and tea polyphenols in the Hui Fen preservatives. The chromatographic conditions were as follow： ZORBAX SB-C18（4.6 mm×250 mm,5 μm） was used, the mobile phase was acetonitrile water,0.1% phosphoric acidmethanol and the flow rate was 0.70 m L / min, at the detection wavelength of 294 nm,and the column temperature was 35 centigrade. The results showed that there was a good linear relationship between the concentration and peak area of aloin and tea polyphenols in the range of 0.044 ~0.264 mg / m L（r =0.999 2） and 0.081 6 ~2.937 6 mg / m L（r =0.999 1）. The average recoveries of aloin was 98.25%,relative standard deviation（RSD） was 2.07% and the average recovery rate of Tea polyphenols was 100.31%,relative standard deviation（RSD） was 1.42%（n=5）. The average content of 5 different batches of aloin in AI Jie preservatives was 0.095 mg / m L,the tea polyphenols was 0.090 mg / m L. The method is simple, accurate and reproducible, can be used to control the content of active ingredient of aloin and tea polyphenol in Hui Fen preservatives.